Simultaneous chiral resolution of monosaccharides as 8-aminonaphthalene-1,3,6-trisulfonate derivatives by ligand-exchange CE using borate as a central ion of the chiral selector
Simultaneous chiral resolution of monosaccharides as 8-aminonaphthalene-1,3,6-trisulfonate derivatives by ligand-exchange CE using borate as a central ion of the chiral selector
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DOI：
10.1002/elps.200700306
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发表时间：
2007-11-01
期刊：
影响因子：
2.9
通讯作者：
Hayakawa, Kazuichi
中科院分区：
文献类型：
--
作者：
Kodama, Shuji;Aizawa, Sen-ichi;Hayakawa, Kazuichi
六种还原单糖（甘露糖、半乳糖、岩藻糖、葡萄糖、木糖和阿拉伯糖）用 8-氨基萘-1,3,6-三磺酸盐 (ANTS) 衍生化。基于以硼酸盐作为手性选择剂中心离子、(S)-3-氨基-1,2-丙二醇(SAP)作为手性选择剂配体的手性配体交换原理，利用245 nm吸光度同时对所有6种ANTS单糖进行对映分离并进行检测。实现高分辨率和适度短迁移时间的最佳条件包括 200 mM SAP-200 mM 硼酸盐缓冲液 (pH 9.2)，其中含有 10% ACN 作为 BGE，温度为 30 摄氏度，外加电压为 +30 kV。研究表明，所提出的使用 SAP-硼酸盐系统的手性配体交换毛细管电泳不仅适用于二醇的对映分离，也适用于多元醇的对映分离。
Six reducing monosaccharides (mannose, galactose, fucose, glucose, xylose, and arabinose) were derivatized with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS). Based on the chiral ligand-exchange principle using borate as a central ion of the chiral selector and (S)-3-amino-1,2-propanediol (SAP) as a chiral selector ligand, all of the six ANTS-monosaccharides were simultaneously enantioseparated using absorbance at 245 nm for detection. The optimum conditions for both high resolution and moderately short migration time consisted of 200 mM SAP-200 mM borate buffer (pH 9.2) containing 10% ACN as a BGE at 30 degrees C with an applied voltage of +30 kV. it was revealed that the proposed chiral ligand-exchange CE using the SAP-borate system was applicable to enantioseparation of not only diols but also polyols.