Identification of residues in fission yeast and human p34cdc2 required for S-M checkpoint control.
Identification of residues in fission yeast and human p34cdc2 required for S-M checkpoint control.
复制标题
鉴定 S-M 检查点控制所需的裂殖酵母和人 p34cdc2 中的残留物。
DOI：
10.1093/genetics/144.4.1413
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Enoch,T
中科院分区：
文献类型：
--
作者：
Basi,G;Enoch,T
在分裂酵母中，p34cdc2的调控在连接有丝分裂到完成DNA复制的检查点过程中起着重要作用。Cdc2突变scdc2-3w(C67Y)和cdc2-4w(C67F)在不严重影响正常细胞增殖的情况下取消了检查点控制。然而，这种表型的分子基础尚不清楚。为了更好地了解p34cdc2在检查点控制中的作用，我们在具有这一表型的裂殖酵母cdc2中筛选了更多的突变。我们已经分离到cdc2-3wand cdc2-4w以及三个新的cdc2等位基因：cdc2-6w(N66I)、cdc2-7w(E8V)和cdc2-8w(K9E)。改变后的残基映射到蛋白质相反面上的两个不同区域，表明p34cdc2与检查点途径组件之间的相互作用可能是复杂的。与cdc2-3wand cdc2-4w不同，新的突变改变了分裂酵母cdc+和其他CDK之间保守的残基，包括人CDC2蛋白。等效人CDC2突变体在裂解酵母中的表达取消了检查点控制，这表明这些残基可能参与了对其他真核CDK的检查点依赖的调控。
In fission yeast, regulation of p34cdc2plays an important role in the checkpoint coupling mitosis to completion of DNA replication. Thecdc2mutationscdc2-3w(C67Y) andcdc2-4w(C67F) abolish checkpoint control without seriously affecting normal cell proliferation. However the molecular basis of this phenotype is not known. To better understand the role of p34cdc2in checkpoint control, we have screened for more mutations inSchizosaccharomyces pombe cdc2with this phenotype. We have isolatedcdc2-3wandcdc2-4w,as well as three newcdc2alleles:cdc2-6w(N66I),cdc2-7w(E8V) andcdc2-8w(K9E). The altered residues map to two different regions on opposite faces of the protein, suggesting that the interaction between p34cdc2and components of the checkpoint pathway may be complex. In contrast tocdc2-3wandcdc2-4w, the new mutations alter residues that are conserved between the fission yeastcdc+and other cdks, including the humanCDC2protein. Expression of the equivalent humanCDC2mutants in fission yeast abolishes checkpoint control, suggesting that these residues could be involved in checkpoint-dependent regulation of other eukaryotic cdks.