Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers.
Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers.
复制标题
DOI：
10.1016/j.msec.2016.08.032
复制
发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
M. Ranjbar-Mohammadi;S. Rabbani;S. Bahrami;M. Joghataei;F. Moayer
中科院分区：
文献类型：
--
作者：
M. Ranjbar-Mohammadi;S. Rabbani;S. Bahrami;M. Joghataei;F. Moayer
在这项研究中，我们描述了静电纺丝姜黄素负载的聚（ε-己内酯）（PCL）/黄芪胶（GT）（PCL/GT/Cur）纳米纤维用于糖尿病大鼠伤口愈合的潜力。这些对耐甲氧西林金黄色葡萄球菌革兰氏阳性菌和广谱β内酰胺酶革兰氏阴性菌具有抗菌性能的支架以无细胞和细胞接种两种形式应用，以评估其愈合大鼠背部全层伤口的能力。 15天后，病理学研究表明，应用GT/PCL/Cur纳米纤维可使伤口明显快速闭合，形成良好的肉芽组织，以成纤维细胞增殖、胶原沉积、完整的早期再生上皮层以及汗腺和毛囊的形成为主。在此期间，未处理的对照中没有观察到这种附属物形成。 Masson 的三色染色证实，第 5 天和第 15 天，经纳米纤维处理的伤口真皮中胶原蛋白含量增加，而对照伤口在第 5 天基本上没有胶原蛋白，并且在第 15 天表现出较少的胶原蛋白含量。第 5 天支架的定量分析证实，与相比，具有增加的血管生成数量、肉芽组织面积 (μ2)、成纤维细胞数量和减小的上皮间隙 (μ) 的组织工程支架更有效。 GT/PCL/Cur 纳米纤维。
In this study we describe the potential of electrospun curcumin-loaded poly(ε-caprolactone) (PCL)/gum tragacanth (GT) (PCL/GT/Cur) nanofibers for wound healing in diabetic rats. These scaffolds with antibacterial property against methicillin resistantStaphylococcus aureusas gram positive bacteria and extended spectrum β lactamase as gram negative bacteria were applied in two forms of acellular and cell-seeded for assessing their capability in healing full thickness wound on the dorsum of rats. After 15 days, pathological study showed that the application of GT/PCL/Cur nanofibers caused markedly fast wound closure with well-formed granulation tissue dominated by fibroblast proliferation, collagen deposition, complete early regenerated epithelial layer and formation of sweat glands and hair follicles. No such appendage formation was observed in the untreated controls during this duration. Masson's trichrome staining confirmed the increased presence of collagen in the dermis of the nanofiber treated wounds on day 5 and 15, while the control wounds were largely devoid of collagen on day 5 and exhibited less collagen amount on day 15. Quantification analysis of scaffolds on day 5 confirmed that, tissue engineered scaffolds with increased amount of angiogenesis number, granulation tissue area (μ2), fibroblast number, and decreased epithelial gap (μ) can be more effective compared to GT/PCL/Cur nanofibers.