Immortalized hemopoietic cells with stem cell properties.
Immortalized hemopoietic cells with stem cell properties.
复制标题
具有干细胞特性的永生化造血细胞。
DOI：
10.1016/1074-7613(94)90047-7
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发表时间：
1994
期刊：
影响因子：
32.4
通讯作者：
Chung,SW
中科院分区：
文献类型：
--
作者：
Wong,PM;Han,XD;Ruscetti,FW;Chung,SW
我们已经建立了一个造血细胞系，BL 3，具有重排的逆转录病毒基因组，我们使用它作为一个遗传标签，他们植入到致命的辐射小鼠。移植后7个月的受体分析表明，标记物存在于各种造血器官的分化细胞中，集落形成细胞中，前CFU-S形成细胞中，以及来自初次受体骨髓细胞的二次受体器官中。BL 3细胞是Thy-I+、Sea-1+、B220-、Mac-i-和Gr-I-。它们表达加塔-1，并能够与基质细胞形成“鹅卵石”。他们不表达和响应几个造血生长因子已知促进骨髓恢复。然而，它们受TGF β负调控，并且可以被有丝分裂原刺激的脾细胞条件培养基刺激。我们的结论是，BL 3细胞具有造血干细胞的特性，包括他们的能力，有助于长期再增殖。
We have established a hemopoietic cell line, BL3, that possesses a rearranged retroviral genome, which we used as a genetic tag for their engraftment into lethally irradiated mice. Analysis of recipients up to 7 months after engraftment indicates that the marker was present in differentiated cells of various hemopoietic organs, in colony-forming cells, pre-CFU-S-forming cells, and in organs of secondary recipients from bone marrow cells of primary recipients. BL3 cells are Thy-I+, Sea-1+, B220-, Mac-i-, and Gr-I-. They express GATA-1 and are able to develop “cobblestones” with stromal cells. They do not express and respond to several hemopoietic growth factors known to facilitate marrow recovery. However, they are negatively regulated by TGFp and can be stimulated by mitogenstimulated spleen cell-conditioned medium. We conclude that BL3 cells possess properties of hemopoietic stem cells, including their capability to contribute to long-term repopulation.