METABOLISM OF 1,2-PROPANEDIOL
METABOLISM OF 1,2-PROPANEDIOL
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DOI：
10.1016/0006-3002(61)90048-8
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发表时间：
1961-01-01
期刊：
影响因子：
--
通讯作者：
HUFF, E
中科院分区：
文献类型：
--
作者：
HUFF, E
Lactaldehyde has been identified as the product of the oxidation of DL-1,2-propanediol by a rabbit-liver alcohol de-hydrogenase preparation. The K3 values for (a) oxidation of ethanol, L-1,2-propanediol, and D-1,2-propanediol are respectively 0.63, 3.6, and 33.3 [mu]moles/ml; and for (b) reduction of acetaldehyde, L-lactaldehyde, and D-lactaldehyde are respectively 3.6, 1.4, and 3.7 [mu]moles/ml. The enzyme was most active in the reduction of L-lactal-dehyde. Studies with crystalline horse-liver alcohol dehydrogenase yielded results similar to those observed with the rabbit-liver enzyme preparation. The K3 values for (a) oxidation of ethanol, L-1,2-propanediol, and D-1,2-propanediol are respectively 2.1, 12.8, and 56 [mu]moles/ml; and for (b) reduction of acetaldehyde, L-lactaldehyde, and D-lactaldehyde are respectively 0.12, 0.12, and 0.26 [mu]mole/ml. It is pointed out that these results demonstrate a new stereospecificity of alcohol dehydrogenase involving the carbon adjacent to the one being oxidized. The importance of using a recently described technique of bisulfite ion exchange chromatography for the separation and identification of acetol and lactaldehyde is discussed.
