Comparative morphology of the osteocyte lacunocanalicular system in various vertebrates
Comparative morphology of the osteocyte lacunocanalicular system in various vertebrates
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DOI：
10.1007/s00774-011-0268-6
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发表时间：
2011-11-01
影响因子：
3.3
通讯作者：
Yamaguchi, Akira
中科院分区：
文献类型：
--
作者：
Cao, Lei;Moriishi, Takeshi;Yamaguchi, Akira
骨细胞嵌入骨基质中，通过骨细胞腔隙管系统与邻近的骨细胞、成骨细胞和破骨细胞交流。骨细胞被认为对维持骨骼稳态至关重要，因为它们调节哺乳动物骨骼中的机械传感和矿物质代谢；然而，其他脊椎动物的骨细胞形态尚未得到很好的记录。我们对以下脊椎动物的骨细胞形态和腔管系统进行了比较研究：两种硬骨鱼[米达卡鱼（Oryzias latipes）和斑马鱼（Danio rerio）]，三种两栖动物[非洲爪蛙（Xenopus laevis），黑斑塘蛙（Rana nigromaculata）和日本火腹蝾螈（Cynops pyrrhogaster）]，两种爬行动物[四趾龟（Testudo horsfieldii）和绿鬣蜥（iguana iguana）]，以及两种哺乳动物（实验小鼠C57BL6和人类）。采用改良银染色法和荧光素偶联法观察骨细胞腔管系统的分布。medaka骨很少有骨细胞（无细胞骨）。斑马鱼的骨骼含有骨细胞，但骨细胞腔管系统发育不全。淡水物种非洲爪蟾（Xenopus laevis）以及其他两栖动物、爬行动物和哺乳动物的骨头都含有大量的骨细胞和发达的腔管系统。本研究表明骨细胞腔管系统的发育在硬骨鱼和陆地脊椎动物之间存在差异，但这种模式与水生栖息地没有直接关系。
Osteocytes are embedded in the bone matrix, and they communicate with adjacent osteocytes, osteoblasts, and osteoclasts through the osteocyte lacunocanalicular system. Osteocytes are believed to be essential for the maintenance of bone homeostasis because they regulate mechanical sensing and mineral metabolism in mammalian bones; however, osteocyte morphology in other vertebrates has not been well documented. We conducted a comparative study on the morphology of osteocytes and the lacunocanalicular system of the following vertebrates: two teleost fishes [medaka (Oryzias latipes), and zebrafish (Danio rerio)], three amphibians [African clawed frog (Xenopus laevis), black-spotted pond frog (Rana nigromaculata), and Japanese fire-bellied newt (Cynops pyrrhogaster)], two reptiles [four-toed tortoise (Testudo horsfieldii) and green iguana (Iguana iguana)], and two mammals (laboratory mouse C57BL6 and human). The distribution of the osteocyte lacunocanalicular system in all these animals was investigated using the modified silver staining and the fluorescein-conjugated phalloidin staining methods. Bones of medaka had few osteocytes (acellular bone). Bones of zebrafish contained osteocytes (cellular bone) but had a poorly developed osteocyte lacunocanalicular system. Bones of Xenopus laevis, a freshwater species, and of other amphibians, reptiles, and mammals contained numerous osteocytes and a well-developed lacunocanalicular system. The present study indicates that development of the osteocyte lacunocanalicular system differs between teleost fishes and land vertebrates, but this pattern is not directly related to aquatic habitat.