Characterisation of mecA gene negative Staphylococcus aureus isolated from bovine mastitis milk from Northern Germany
Characterisation of mecA gene negative Staphylococcus aureus isolated from bovine mastitis milk from Northern Germany
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DOI：
10.1007/s12223-019-00698-z
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发表时间：
2019-11-01
影响因子：
2.6
通讯作者：
Abdulmawjood, A.
中科院分区：
文献类型：
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作者：
Sheet, O. H.;Grabowski, N. T.;Abdulmawjood, A.
金黄色葡萄球菌（S. aureus）是奶牛传染性乳间感染的重要病原体。金黄色葡萄球菌也被认为是一种重要的食源性病原体，也是世界范围内食物中毒病例和爆发的原因。为了了解金黄色葡萄球菌的分子生态学，本研究比较了2001年8月至2014年3月在德国北部不同地区采集的70株牛乳腺炎乳样品中的金黄色葡萄球菌的表型和基因型特征。利用多位点序列分型（MLST）、spa分型以及编码16种葡萄球菌肠毒素（sea-selu）、中毒性休克综合征毒素（tst）、热核酸酶（nuc）、凝块因子（clfA和clfB）、凝固酶（coa）和甲氧西林耐药基因mecA的毒力基因，对金黄色葡萄球菌分离株进行了表型和基因表型的特征分析。共将16个序列类型划分为8个克隆复合物（CCs），鉴定出17个spa类型。其中包括6种新的序列类型和1种新的spa类型。大多数奶牛乳腺炎乳相关序列类型属于克隆复合体CC5、CC97、CC133和CC151，与人类来源的序列类型表现出密切相关的基因型或谱系。基因型以CC133 （ST133-t1403）为主，占总数的27.1%。此外，金黄色葡萄球菌分离株显示出9种不同的肠毒素生成谱。所有金黄色葡萄球菌对甲氧西林敏感（MSSA）。本研究为牛乳腺炎金黄色葡萄球菌分离株的表型和基因型特征提供了新的信息。本研究从乳腺炎乳汁中分离出的分离株的特征比较显示与人类分离株相似。这可能有助于更好地了解金黄色葡萄球菌在同一健康环境中的分布。
Staphylococcus aureus (S. aureus) is an important causative agent of contagious intermammary infections in dairy cattle. S. aureus is also considered as an important foodborne pathogen and cause of food poisoning cases and outbreaks worldwide. In order to understand the molecular ecology of S. aureus, the present study compared phenotypic and genotypic characteristics of 70 S. aureus isolates from bovine mastitis milk samples collected during the period from August 2001 to March 2014 in different regions of Northern Germany. The S. aureus isolates were characterised phenotypically, as well as genotypically for their genetic diversity using multi-locus sequence typing (MLST), spa typing and the presence of virulence genes encoding 16 staphylococcal enterotoxins (sea-selu), toxic shock syndrome toxin (tst), thermonuclease (nuc), clumping factor (clfA and clfB), coagulase (coa) and the methicillin resistance gene mecA. A total of 16 sequence types were grouped into eight clonal complexes (CCs), and 17 spa types were identified. These included six novel sequence types and one novel spa type. The majority of bovine mastitis milk-associated sequence types belonged to the clonal complex CC5, CC97, CC133, and CC151 and showed closely related genotypes or lineages with sequence types of human origin. The genotype CC133 (ST133-t1403) was predominant, constituting 27.1% of the isolates. In addition, the S. aureus isolates displayed nine different enterotoxigenic profiles. All S. aureus were methicillin susceptible (MSSA). The current study provides new information on phenotypic and genotypic traits of S. aureus isolates from bovine mastitis. The comparison of characteristics of isolates from the present study originating from mastitis milk showed similarities with human isolates. This might help to better understand the distribution of S. aureus in the one health context.