Repeated Exposure to High-Frequency Low-Amplitude Vibration Induces Degeneration of Murine Intervertebral Discs and Knee Joints
Repeated Exposure to High-Frequency Low-Amplitude Vibration Induces Degeneration of Murine Intervertebral Discs and Knee Joints
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DOI：
10.1002/art.39154
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发表时间：
2015-08-01
影响因子：
13.3
通讯作者：
Seguin, Cheryle A.
中科院分区：
文献类型：
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作者：
McCann, Matthew R.;Patel, Priya;Seguin, Cheryle A.
Objective.高频、低振幅全身振动（WBV）正被用于治疗一系列肌肉骨骼疾病;然而，关于其对关节组织的影响的知识却令人惊讶地有限。本研究通过小鼠体内模型研究了反复暴露于WBV对骨和关节组织的影响。将10周龄的雄性小鼠暴露于模拟人类临床使用的条件下的垂直正弦振动（每天30分钟，每周5天，45 Hz，峰值加速度为0.3g）。WBV后，通过显微计算机断层扫描、组织学分析和免疫组化检查骨骼组织，并使用实时聚合酶链反应定量基因表达。WBV治疗4周后，椎间盘显示纤维环变性、胶原组织破坏和细胞死亡增加的组织学标志。与对照组相比，暴露于WBV的小鼠椎间盘中Mmp 3表达增加，伴随着胶原蛋白和聚集蛋白聚糖降解增强。WBV治疗4周后膝关节检查显示关节软骨撕裂和局灶性损伤，类似骨关节炎的变化。此外，与对照组相比，暴露于WBV的小鼠还表现出更高的Mmp 13基因表达，并增强了关节软骨中基质金属蛋白酶介导的胶原蛋白和聚集蛋白聚糖降解。胫骨近端骨小梁微结构和密度无明显变化。我们的实验揭示了WBV对小鼠模型关节组织的显著负面影响。这些发现表明，需要进一步研究WBV对人类关节健康的影响。
Objective. High-frequency, low-amplitude whole-body vibration (WBV) is being used to treat a range of musculoskeletal disorders; however, there is surprisingly limited knowledge regarding its effect(s) on joint tissues. This study was undertaken to examine the effects of repeated exposure to WBV on bone and joint tissues in an in vivo mouse model.Methods. Ten-week-old male mice were exposed to vertical sinusoidal vibration under conditions that mimic those used clinically in humans (30 minutes per day, 5 days per week, at 45 Hz with peak acceleration at 0.3g). Following WBV, skeletal tissues were examined by micro-computed tomography, histologic analysis, and immunohistochemistry, and gene expression was quantified using real-time polymerase chain reaction.Results. Following 4 weeks of WBV, intervertebral discs showed histologic hallmarks of degeneration in the annulus fibrosus, disruption of collagen organization, and increased cell death. Greater Mmp3 expression in the intervertebral disc, accompanied by enhanced collagen and aggrecan degradation, was found in mice exposed to WBV as compared to controls. Examination of the knee joints after 4 weeks of WBV revealed meniscal tears and focal damage to the articular cartilage, changes resembling osteoarthritis. Moreover, mice exposed to WBV also demonstrated greater Mmp13 gene expression and enhanced matrix metalloproteinase-mediated collagen and aggrecan degradation in articular cartilage as compared to controls. No changes in trabecular bone microarchitecture or density were detected in the proximal tibia.Conclusion. Our experiments reveal significant negative effects of WBV on joint tissues in a mouse model. These findings suggest the need for future studies of the effects of WBV on joint health in humans.