Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori
Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori
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DOI：
10.1111/j.1365-2583.2010.01020.x
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发表时间：
2010-10-01
影响因子：
2.6
通讯作者：
Shimada, T.
中科院分区：
文献类型：
--
作者：
Fujii, T.;Daimon, T.;Shimada, T.
家蚕幼虫的体壁是不透明的，因为尿酸盐颗粒积聚在表皮中。虽然尿酸的生物合成途径已被充分研究，但对尿酸如何在表皮细胞中积累为尿酸盐颗粒知之甚少。在独特的油（od）突变体家蚕，幼虫体壁是半透明的，因为不能构建尿酸颗粒。最近，我们发现od突变体在B区有一个基因组缺失。家蚕同源的人类生物合成溶酶体相关细胞器复合体1，亚基2（BLOS2）基因（BmBLOS2）。在这里，我们进行了BmBL0S2的分子和功能表征。北方印迹分析表明BmBLOS 2在多种组织中广泛表达。我们分析了一个新分离的突变体（odB）等位基因OD的结构，并发现在这个新的突变BmBLOS 2的编码序列中的提前终止密码子。此外，半透明表型被拯救的野生型BmBL0S2等位基因到od突变体的种系转化。我们的研究结果表明，BmBLOS2是负责od突变表型，并发挥了至关重要的作用，在幼虫表皮尿酸盐颗粒的生物合成。哺乳动物HPS基因、果蝇颗粒群基因和B半透明突变基因之间的关系。森是讨论。
The larval integument of the silkworm, Bombyx mori, is opaque because urate granules accumulate in the epidermis. Although the biosynthetic pathway of uric acid is well studied, little is known about how uric acid accumulates as urate granules in epidermal cells. In the distinct oily (od) mutant silkworm, the larval integument is translucent because of the inability to construct urate granules. Recently, we have found that the od mutant has a genomic deletion in the B. mori homologue of the human biogenesis of lysosome-related organelles complex1, subunit 2 (BLOS2) gene (BmBLOS2). Here, we performed a molecular and functional characterization of BmBLOS2. Northern blot analysis showed that BmBLOS2 was ubiquitously expressed in various tissues. We analysed the structure of a newly isolated mutant (odB) allelic to od and found a premature stop codon in the coding sequence of BmBLOS2 in this new mutation. Moreover, the translucent phenotype was rescued by the germ-line transformation of the wild-type BmBLOS2 allele into the od mutant. Our results suggest that BmBLOS2 is responsible for the od mutant phenotype and plays a crucial role in biogenesis of urate granules in the larval epidermis of the silkworm. The relationships amongst Hermansky-Pudlak syndrome (HPS) genes in mammals, granule group genes in Drosophila and translucent mutant genes in B. mori are discussed.