EXPRESSION AND SECRETION OF TYPE-BETA TRANSFORMING GROWTH-FACTOR BY ACTIVATED HUMAN MACROPHAGES
EXPRESSION AND SECRETION OF TYPE-BETA TRANSFORMING GROWTH-FACTOR BY ACTIVATED HUMAN MACROPHAGES
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DOI：
10.1073/pnas.84.17.6020
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发表时间：
1987-09-01
影响因子：
11.1
通讯作者：
SPORN, MB
中科院分区：
文献类型：
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作者：
ASSOIAN, RK;FLEURDELYS, BE;SPORN, MB
用刀豆球蛋白A激活的肺泡巨噬细胞和用脂多糖激活的外周血单核细胞分泌β型。转化生长因子（TGF-β）。 TGF-β 含量极少。未激活的单核细胞中的分泌，即使TGF-β。 mRNA 在这些细胞中的表达水平与活化的脂多糖处理的培养物中的表达水平相似。具有单核细胞特征的U937淋巴瘤细胞也表达TGF-β的mRNA。新鲜分离的单核细胞，对照和脂多糖处理的，分泌抑制TGF-β的酸不稳定结合蛋白。行动。我们得出以下结论：(i)TGF-β的表达。 mRNA 与单核细胞活化无关，(ii) TGF-β 的分泌。由单核细胞活化诱导，并且(iii) TGF-β的共同分泌。其单核细胞/巨噬细胞衍生的结合蛋白可能调节生长因子的作用。相比之下，其他生长因子基因（例如血小板衍生生长因子的 B 链）的单核表达不是组成型的，需要激活。
Alveolar macrophages activated with concanavalin A and peripheral blood monocytes activated with lipopolysaccharide secrete type .beta. transforming growth factor (TGF-.beta.). There is minimal TGF-.beta. secretion in unactivated monocytes, even though TGF-.beta. mRNA is expressed in these cells at a level similar to that in activated, lipopolysaccharide-treated cultures. U937 lymphoma cells, which have monocytic characteristics, also express mRNA for TGF-.beta.. Freshly isolated monocytes, both control and lipopolysaccharide-treated, secrete an acid-labile binding protein that inhibits TGF-.beta. action. We conclude the following: (i) that expression of TGF-.beta. mRNA is unrelated to monocyte activation, (ii) that secretion of TGF-.beta. is induced by monocyte activation, and (iii) that cosecretion of TGF-.beta. and its monocyte/macrophage-derived binding protein may modulate growth factor action. In contrast, monocytic expression of other growth factor genes, such as the B chain of platelet-derived growth factor, is not constitutive and requires activation.