Transcriptomic profiling of Trypanosoma congolense mouthpart parasites from naturally infected flies.
Transcriptomic profiling of Trypanosoma congolense mouthpart parasites from naturally infected flies.
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DOI：
10.1186/s13071-022-05258-y
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发表时间：
2022-05-02
影响因子：
3.2
通讯作者：
Jackson, Andrew
中科院分区：
文献类型：
--
作者：
Pereira, Sara Silva;Mathenge, Kawira;Masiga, Daniel;Jackson, Andrew
动物非洲锥虫病，或nagana，是一种由采采蝇传播的非洲锥虫引起的兽医疾病。在非洲，刚果锥虫是牲畜中最具致病性和最普遍的病原之一，导致动物高发病率和死亡率以及广泛的生产损失。在采采蝇中，寄生虫在中肠定居，并最终到达口器，当苍蝇以脊椎动物宿主（如牛）为食时，它们可以从那里传播。尽管口器形式的寄生虫对疾病传播的极端重要性，但在这些寄生虫形式中进行的全球表达谱研究很少。在这里，我们收集了来自辛巴山国家保护区的采采蝇，这是肯尼亚东南部的一个野生动物保护区。congolense感染，并对T.寄生在苍蝇口器上的刚果寄生虫。我们发现自然和实验苍蝇感染的口器寄生虫之间几乎没有相关性。此外，我们进行了差异基因表达分析之间的口器和血流寄生虫的形式，并确定了几个表面表达的基因和152个新的假设蛋白质差异表达的口器寄生虫。最后，我们分析了变异抗原的表达，并观察到一个变异表面糖蛋白（VSG）转录本属于T。先前观察到在后环转录组中富集的刚果绦虫8型（即TcIL3000.A.H_000381200）存在于所有野生捕获的口器样品以及血流形式的寄生虫中，提示组成型表达。我们的研究提供了从自然感染采采蝇锥虫寄生虫的转录组，并表明，一个8型VSG基因组成型表达的代谢和血流形式的寄生虫在人口水平。在线版本包含补充材料，可通过10.1186/s13071-022-05258-y获得。
Animal African trypanosomiasis, or nagana, is a veterinary disease caused by African trypanosomes transmitted by tsetse flies. In Africa, Trypanosoma congolense is one of the most pathogenic and prevalent causes of nagana in livestock, resulting in high animal morbidity and mortality and extensive production losses. In the tsetse fly, parasites colonise the midgut and eventually reach the mouthparts, from where they can be transmitted as the fly feeds on vertebrate hosts such as cattle. Despite the extreme importance of mouthpart-form parasites for disease transmission, very few global expression profile studies have been conducted in these parasite forms. Here, we collected tsetse flies from the Shimba Hills National Reserve, a wildlife area in southeast Kenya, diagnosed T. congolense infections, and sequenced the transcriptomes of the T. congolense parasites colonising the mouthparts of the flies. We found little correlation between mouthpart parasites from natural and experimental fly infections. Furthermore, we performed differential gene expression analysis between mouthpart and bloodstream parasite forms and identified several surface-expressed genes and 152 novel hypothetical proteins differentially expressed in mouthpart parasites. Finally, we profiled variant antigen expression and observed that a variant surface glycoprotein (VSG) transcript belonging to T. congolense phylotype 8 (i.e. TcIL3000.A.H_000381200), previously observed to be enriched in metacyclic transcriptomes, was present in all wild-caught mouthpart samples as well as bloodstream-form parasites, suggestive of constitutive expression. Our study provides transcriptomes of trypanosome parasites from naturally infected tsetse flies and suggests that a phylotype 8 VSG gene is constitutively expressed in metacyclic- and bloodstream-form parasites at the population level. The online version contains supplementary material available at 10.1186/s13071-022-05258-y.
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影响因子：
3.2
作者：
Ebhodaghe, Faith I.;Bastos, Armanda D. S.;Okal, Michael N.
通讯作者：
Okal, Michael N.
影响因子：
3.2
作者：
Gachoki S;Groen T;Vrieling A;Okal M;Skidmore A;Masiga D
通讯作者：
Masiga D
影响因子：
3.3
作者：
Abbas AH;Silva Pereira S;D'Archivio S;Wickstead B;Morrison LJ;Hall N;Hertz-Fowler C;Darby AC;Jackson AP
通讯作者：
Jackson AP
影响因子：
46.9
作者：
Bray, Nicolas L.;Pimentel, Harold;Pachter, Lior
通讯作者：
Pachter, Lior
DOI：
10.1073/pnas.1117313109
发表时间：
2012-02-28
影响因子：
11.1
作者：
Jackson, Andrew P.;Berry, Andrew;Berriman, Matthew
通讯作者：
Berriman, Matthew