The susceptibility of pure tubulin to high magnetic fields:: A magnetic birefringence and x-ray fiber diffraction study
The susceptibility of pure tubulin to high magnetic fields:: A magnetic birefringence and x-ray fiber diffraction study
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DOI：
10.1016/s0006-3495(98)77863-4
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发表时间：
1998-03-01
影响因子：
3.4
通讯作者：
Medrano, FJ
中科院分区：
文献类型：
--
作者：
Bras, W;Diakun, GP;Medrano, FJ
研究了微管在强磁场下的取向行为。结果表明，当微管在磁场中组装时，它们的长轴与磁场平行排列。已知的影响微管装配的几个参数的影响，研究了它们对最终对准程度的影响。制备了适合低分辨率x射线光纤衍射实验的水合微管排列样品，并将光纤衍射实验结果与磁双折射实验结果进行了比较。并与早期的光纤衍射工作和小角x射线溶液散射实验进行了比较。
The orientational behavior of microtubules assembled in strong magnetic fields has been studied. It is shown that when microtubules are assembled in a magnetic field, they align with their long axis parallel to the magnetic field. The effect of several parameters known to affect the microtubule assembly are investigated with respect to their effect on the final degree of alignment. Aligned samples of hydrated microtubules suitable for low-resolution x-ray fiber diffraction experiments have been produced, and the results obtained from the fiber diffraction experiments have been compared with the magnetic birefringence experiments. Comparisons with earlier fiber diffraction work and small-angle x-ray solution scattering experiments have been made.