Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds
Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds
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DOI：
10.1111/j.1524-475x.2007.00351.x
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发表时间：
2008-05-01
影响因子：
2.9
通讯作者：
Nanney, Lillian B.
中科院分区：
文献类型：
--
作者：
Caldwell, Robert L.;Opalenik, Susan R.;Nanney, Lillian B.
MRL/MpJ-Fas（lpr）小鼠表现出再生通过真皮穿孔切除的耳组织的能力。这是一个特殊的模型，以确定候选蛋白质，可能会调节再生，在典型的非再生组织。鉴定参与再生的关键分子可以拓宽我们对伤口愈合过程的理解，并产生新的治疗方法。基质辅助激光解吸电离质谱法的组织分析是一种快速、强大的蛋白质组学工具，可以从完整组织标本的特定区域检测数百种蛋白质。为了鉴定这些候选分子，使用MRL/MpJ-Fas（lpr）小鼠和非再生小鼠品系C57 BL/6 J的组织分析，在4天和7天后检查耳穿孔器中的蛋白质表达。光谱分析显示再生和非再生表型之间的蛋白质组差异，包括钙结合蛋白钙颗粒蛋白A和B，calgizzarin和钙调蛋白。这些差异表达的蛋白质的损伤区域内的空间分布，证实了免疫组织化学。
MRL/MpJ-Fas(lpr) mice exhibit the ability to regenerate ear tissue excised by dermal punches. This is an exceptional model to identify candidate proteins that may regulate regeneration in typically nonregenerative tissues. Identification of key molecules involved in regeneration can broaden our understanding of the wound-healing process and generate novel therapeutic approaches. Tissue profiling by matrix-assisted laser desorption ionization mass spectrometry is a rapid, powerful proteomic tool that allows hundreds of proteins to be detected from specific regions of intact tissue specimens. To identify these candidate molecules, protein expression in ear punches was examined after 4 and 7 days using tissue profiling of MRL/MpJ-Fas(lpr) mice and the nonregenerative mouse strain C57BL/6J. Spectral analysis revealed distinct proteomic differences between the regenerative and nonregenerative phenotypes, including the calcium-binding proteins calgranulin A and B, calgizzarin, and calmodulin. Spatial distributions for these differentially expressed proteins within the injured regions were confirmed by immunohistochemistry.