ANALYSIS OF XANTHOPHYLL CYCLE CAROTENOIDS AND CHLOROPHYLL FLUORESCENCE IN LIGHT INTENSITY-DEPENDENT CHLOROPHYLL-DEFICIENT MUTANTS OF WHEAT AND BARLEY
ANALYSIS OF XANTHOPHYLL CYCLE CAROTENOIDS AND CHLOROPHYLL FLUORESCENCE IN LIGHT INTENSITY-DEPENDENT CHLOROPHYLL-DEFICIENT MUTANTS OF WHEAT AND BARLEY
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DOI：
10.1007/bf00018262
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发表时间：
1994-12-01
影响因子：
3.7
通讯作者：
ADAMS, WW
中科院分区：
文献类型：
--
作者：
FALBEL, TG;STAEHELIN, LA;ADAMS, WW
在两种不同生长的PFDs（30和600 μ mol光子m（-2）s（-1）入射光）下，分析了三种依赖于光强的叶绿素b缺陷突变体（两种小麦突变体和一种大麦突变体）的叶黄素循环类胡萝卜素和叶绿素荧光特性。在弱光下生长的突变体具有较低水平的总叶绿素和类胡萝卜素每单位叶面积相比，野生型植物，但两者的相对比例没有显着变化菌株之间。相比之下，在强光下生长的突变体具有低得多的叶绿素水平，导致突变体中的类胡萝卜素与叶绿素的比率与野生型相比显着增加。在弱光条件下，叶黄素循环的类胡萝卜素包括约15%的总类胡萝卜素在所有菌株;在强光下的叶黄素循环池增加到超过30%的总类胡萝卜素在野生型植物和超过50%的总类胡萝卜素在三个突变株。而叶黄素循环仍然相当环氧化在低光下生长的所有植物中，植物生长在强光下表现出相当程度的叶黄素循环转化成花药黄质和玉米黄质在昼夜循环，几乎完全转换（超过90%）只发生在突变体。50至95%的叶黄素循环被保留为花药黄质和玉米黄质过夜在这些突变体中，也表现出持续的抑郁症的PS II光化学效率（F-V/F-m），这可能是由于持续高水平的光保护能量耗散活动。叶绿素b缺陷突变体中相对较大的叶黄素循环池可能部分来自内部天线复合物中叶黄素循环的报告浓度，因为叶绿素b缺陷突变体在外周LHC-II复合物中存在缺陷。
Three light intensity-dependent Chl b-deficient mutants, two in wheat and one in barley, were analyzed for their xanthophyll cycle carotenoids and Chl fluorescence characteristics under two different growth PFDs (30 versus 600 mu mol photons m(-2) s(-1) incident light). Mutants grown under low light possessed lower levels of total Chls and carotenoids per unit leaf area compared to wild type plants, but the relative proportions of the two did not vary markedly between strains. In contrast, mutants grown under high light had much lower levels of Chl, leading to markedly greater carotenoid to Chl ratios in the mutants when compared to wild type. Under low light conditions the carotenoids of the xanthophyll cycle comprised approximately 15% of the total carotenoids in all strains; under high light the xanthophyll cycle pool increased to over 30% of the total carotenoids in wild type plants and to over 50% of the total carotenoids in the three mutant strains. Whereas the xanthophyll cycle remained fairly epoxidized in all plants grown under low light, plants grown under high light exhibited a considerable degree of conversion of the xanthophyll cycle into antheraxanthin and zeaxanthin during the diurnal cycle, with almost complete conversion (over 90%) occurring only in the mutants. 50 to 95% of the xanthophyll cycle was retained as antheraxanthin and zeaxanthin overnight in these mutants which also exhibited sustained depressions in PS II photochemical efficiency (F-V/F-m), which may have resulted from a sustained high level of photoprotective energy dissipation activity. The relatively larger xanthophyll cycle pool in the Chl b-deficient mutant could result in part from the reported concentration of the xanthophyll cycle in the inner antenna complexes, given that the Chl b-deficient mutants are deficient in the peripheral LHC-II complexes.