Large Variabilities in host strain susceptibility and phage host range govern interactions between lytic marine phages and their Flavobactetium hosts
Large Variabilities in host strain susceptibility and phage host range govern interactions between lytic marine phages and their Flavobactetium hosts
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DOI：
10.1128/aem.01399-07
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发表时间：
2007-11-01
影响因子：
4.4
通讯作者：
Riemann, Lasse
中科院分区：
文献类型：
--
作者：
Holmfeldt, Karin;Middelboe, Mathias;Riemann, Lasse
噬菌体是海洋浮游细菌的主要致死因子，被认为通过宿主特异性感染和裂解来调节细菌群落组成。在目前的研究中，我们证明了海洋噬菌体-宿主组合的相互作用是复杂的，感染和14裂解的特异性和效率在感染同一细菌种类菌株的噬菌体之间是高度变化的。对瑞典和丹麦沿海水域的23株拟杆菌和46株噬菌体进行了分析。根据基因型和表型分析，其中21株可认为是baltica Cellulophaga （Flavobacteriaceae）菌株。然而，所有菌株都表现出独特的噬菌体敏感性模式，对相同滴度的噬菌体的敏感性差异高达6个数量级。分离出的噬菌体在基因组大小（8 ~ 242kb）和宿主范围（感染1 ~ 20种菌株）上表现出明显的差异。我们的数据表明，海洋浮游细菌对多种共存的噬菌体敏感，对噬菌体感染的敏感性是菌株特异性的，存在于高度敏感和耐药之间，这意味着一个极其复杂的噬菌体-宿主相互作用网络。因此，在菌株鉴定无法解决的研究中，即基于16S rRNA基因或内部转录间隔序列提供的系统发育分辨率的研究中，可能无法检测到噬菌体对浮游细菌群落组成和动态的影响。
Phages are a main mortality factor for marine bacterioplankton and are thought to regulate bacterial community composition through host-specific infection and lysis. In the present study we demonstrate for a marine phage-host assemblage that interactions are complex and that specificity and efficiency of infection and 14 lysis are highly variable among phages infectious to strains of the same bacterial species. Twenty-three Bacteroidetes strains and 46 phages from Swedish and Danish coastal waters were analyzed. Based on genotypic and phenotypic analyses, 21 of the isolates could be considered strains of Cellulophaga baltica (Flavobacteriaceae). Nevertheless, all bacterial strains showed unique phage susceptibility patterns and differed by up to 6 orders of magnitude in sensitivity to the same titer of phage. The isolated phages showed pronounced variations in genome size (8 to >242 kb) and host range (infecting I to 20 bacterial strains). Our data indicate that marine bacterioplankton are susceptible to multiple co-occurring phages and that sensitivity towards phage infection is strain specific and exists as a continuum between highly sensitive and resistant, implying an extremely complex web of phage-host interactions. Hence, effects of phages on bacterioplankton community composition and dynamics may go undetected in studies where strain identify is not resolvable, i.e., in studies based on the phylogenetic resolution provided by 16S rRNA gene or internal transcribed spacer sequences.