Wnt-10b promotes differentiation skin epithelial cells in vitro
Wnt-10b promotes differentiation skin epithelial cells in vitro
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DOI：
10.1016/j.bbrc.2006.01.104
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发表时间：
2006-03-31
影响因子：
3.1
通讯作者：
Ishizaka, S
中科院分区：
文献类型：
--
作者：
Ouji, Y;Yoshikawa, M;Ishizaka, S
为了评估 Wnt-10b 在上皮分化中的作用，我们研究了 Wnt-10b 对成年小鼠来源的原代皮肤上皮细胞 (MPSEC) 的影响。利用基因工程技术制备重组Wnt-10b蛋白（rWnt-10b），并在其存在下培养MPSEC，导致其形态从立方体变为纺锤形，并抑制其增殖。此外，还观察到经典 Wnt 信号通路的参与。经过 rWnt-10b 处理的 MPSEC 在 Ayoub Shklar 染色和免疫细胞化学结果中显示出毛干和毛囊内根鞘的特征。此外，细胞表达分化上皮细胞的 mRNA，包括角蛋白 1、角蛋白 2、loricrin、mHa5 和 mHb5，与基底细胞标记物角蛋白 5 的表达降低相关。这些结果表明 Wnt-10b 促进 MPSEC 的分化。 (c) 2006 Elsevier Inc. 保留所有权利。
To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which resulted in morphological changes from cuboidal to spindle-shaped and inhibited their proliferation. Further, involvement of the canonical Wnt signal pathway was also observed. MPSEC treated with rWnt-10b showed characteristics of the hair shaft and inner root sheath of the hair follicle, in results of Ayoub Shklar staining and immunocytochemistry. Further, the cells expressed mRNA for differentiated epithelial cells, including keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5. These results suggest that Wnt-10b promotes the differentiation of MPSEC. (c) 2006 Elsevier Inc. All rights reserved.