Molecular characterization, expression analysis, and ontogeny of complement component C9 in southern catfish (Silurus meridionalis)
Molecular characterization, expression analysis, and ontogeny of complement component C9 in southern catfish (Silurus meridionalis)
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南方鲶 (Silurus meridionalis) 补体成分 C9 的分子特征、表达分析和个体发育
DOI：
10.1016/j.fsi.2018.11.069
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发表时间：
2019-03-01
影响因子：
4.7
通讯作者：
Hou, Ting-Long
中科院分区：
文献类型：
--
作者：
Fu, Yao-Wu;Zhu, Cheng-Ke;Hou, Ting-Long
补体系统在宿主防御入侵微生物中起重要作用。补体成分C9是参与靶细胞表面上膜攻击复合物（MAC）形成的最后一种成分。本研究从南方鲇（Silurus spinonalis）中克隆了C9基因的全长cDNA序列，其开放阅读框（ORF）为1809 bp，全长为1984 bp。推导的氨基酸序列与其他硬骨鱼相似。C9的mRNA表达在肝、脾、胃、肠和头肾中检测到，在肝中检测到最高水平。利用全胚原位杂交技术，在受精后34 h的卵黄合胞体层中首次检测到C9的mRNA，在出壳后36 h的肝脏中检测到C9的mRNA。C9基因mRNA表达量在注射气单胞菌后的肝、脾和肠中显著上调，表明C9在南方鲇防御入侵病原体中起重要作用。因此，这些结果为了解C9在鱼类早期发育中的功能提供了重要信息。
The complement system plays an important role in host defense against invading microorganisms. Complement component C9 is the last component that is involved in the formation of the membrane attack complex (MAC) on the surface of target cells. In the present study, the full length C9 cDNA sequence of 1984 bp with an open reading frame (ORF) of 1809 bp was cloned from southern catfish (Silurus meridionalis). The deduced amino acid sequence showed similarity with other teleost fish. The mRNA expression of C9 was detected in the liver, spleen, stomach, intestine, and head kidney, with highest levels detected in the liver. The mRNA of C9 was first detected in the yolk syncytial layer at 34 h post fertilization (hpf) with whole mount in situ hybridization, followed by the liver at 36 h post hatching (hph). The mRNA expression of C9 was upregulated significantly in the liver, spleen, and intestine following the injection with Aeromonas hydrophila, suggesting that C9 played an important role in defense against invading pathogens in southern catfish. Therefore, these results provide important information to understand the functions of C9 during fish early development in fish.