Highly sensitive electrochemical detection of potential cytotoxicity of CdSe/ZnS quantum dots using neural cell chip
Highly sensitive electrochemical detection of potential cytotoxicity of CdSe/ZnS quantum dots using neural cell chip
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DOI：
10.1016/j.bios.2011.12.035
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发表时间：
2012-02-15
影响因子：
12.6
通讯作者：
Choi, Jeong-Woo
中科院分区：
文献类型：
--
作者：
Kim, Tae-Hyung;El-Said, Waleed Ahmed;Choi, Jeong-Woo
细胞芯片是近年来发展起来的一种简单、高灵敏度的评估各种化学品或纳米材料毒性的工具。在这里，我们报道了新发现的极低浓度的硫化镉/硫化锌量子点对神经癌细胞细胞内氧化还原环境的潜在细胞毒性效应，这是只有细胞芯片技术才能检测到的。经台盼蓝和差示脉冲伏安法(DPV)检测，以半胱胺(CA)或硫代乙醇酸(TA)为封端的绿色(直径2.1 nm)和红色(直径6.3 nm)量子点在100微克/毫升时具有毒性。然而，在存在浓度依赖性细胞毒性的情况下，传统的四甲基偶氮唑蓝比色法检测不到低浓度(1~10µg/mL)的TA修饰的量子点对神经细胞的毒性作用时，才用DPV法检测其对神经细胞的毒性作用。用四甲基偶氮唑蓝比色法检测，红色和绿色TA量子点分别在10mU g/m L和5 m u g/m L时使细胞产生的电化学信号减弱，而细胞存活率在100 m u g/m L和50 m u g/m L时分别降低。用四甲基偶氮唑蓝比色法检测，5~50mU g/mLRed-TA量子点和5~30mU g/mLGreen-TA量子点处理细胞后，细胞活力的相对下降分别为15%和11.9%。而在相同浓度范围内，DPV信号分别下降了37.5%和39.2%。这意味着电池的氧化还原环境比其他成分更敏感，即使在低浓度下也很容易受到CdSe/ZnS量子点的影响。因此，我们提出的神经细胞芯片可以简单而准确地应用于检测各种分子显像剂的潜在细胞毒性。(C)2011爱思唯尔B.V.保留所有权利。
Cell chip was recently developed as a simple and highly sensitive tool for the toxicity assessment of various kinds of chemicals or nano-materials. Here, we report newly discovered potential cytotoxic effects of CdSe/ZnS quantum dots (QDs) on intracellular redox environment of neural cancer cells at very low concentrations which can be only detected by cell chip technology. Green (2.1 nm in diameter) and red (6.3 nm in diameter) QDs capped with cysteamine (CA) or thioglycolic acid (TA) were found to be toxic at 100 mu g/mL when assessed by trypan blue and differential pulse voltammetry (DPV). However, in case of concentration-dependent cytotoxicity, toxic effects of TA-capped QDs on human neural cells were only measured by DPV method when conventional MTT assay did not show toxicity of TA-capped QDs at low concentrations (1-10 mu g/mL). Red-TA QDs and Green-TA QDs were found to decrease electrochemical signals from cells at 10 mu g/mL and 5 mu g/mL, respectively, while cell viability decreased at 100 mu g/mL and 50 mu g/mL when assessed by MTT assay, respectively. The relative decreases of cell viability determined by MTT assay were 15% and 11.9% when cells were treated with 5-50 mu g/mL of Red-TA QDs and 5-30 mu g/mL of Green-TA QDs, respectively. However, DPV signals decreased 37.5% and 39.2% at the same concentration range, respectively. This means that redox environment of cells is more sensitive than other components and can be easily affected by CdSe/ZnS QDs even at low concentrations. Thus, our proposed neural cell chip can be applied to detect potential cytotoxicity of various kinds of molecular imaging agents simply and accurately. (C) 2011 Elsevier B.V. All rights reserved.