The Outer Membrane Protein OmpW Enhanced V. cholerae Growth in Hypersaline Conditions by Transporting Carnitine
The Outer Membrane Protein OmpW Enhanced V. cholerae Growth in Hypersaline Conditions by Transporting Carnitine
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外膜蛋白 OmpW 通过转运肉碱增强霍乱弧菌在高盐条件下的生长
DOI：
10.3389/fmicb.2017.02703
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发表时间：
2018-01-22
影响因子：
5.2
通讯作者：
Kan, Biao
中科院分区：
文献类型：
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作者：
Fu, Xiuping;Zhang, Jingyun;Kan, Biao
致病性海洋细菌存在于盐浓度高的环境和食物来源中，细菌必须对其生存进行有效管理。离子和相容溶质的运输以及有氧和无氧呼吸的变化等多种机制赋予细菌耐盐性。在这项研究中，我们发现霍乱弧菌的外膜蛋白OmpW与盐胁迫有关，并且ompW基因转录和表达在含有高浓度NaCl的培养物中上调。 ompW 的缺失导致高盐培养条件下霍乱弧菌的生长减少。补充相容性溶质甜菜碱、L-肉碱或L-赖氨酸可增强霍乱弧菌在高盐介质中的生长。甜菜碱或L-赖氨酸的补充对在高盐培养基中培养的ompW缺失突变体具有相同的生长促进作用，而L-肉碱补充不能恢复突变体的生长。此外，ompW 缺失突变体对左旋肉碱的摄取也有所减少。我们的研究表明，在增强霍乱弧菌高盐耐受性的多重因素中，OmpW还通过转运左旋肉碱发挥作用。
Pathogenic marine bacteria are found in environments and food sources with high salt concentrations, which the bacteria must effectively manage for their survival. Several mechanisms, such as the transport of ions and compatible solutes as well as changes in aerobic and anaerobic respiration, confer salt tolerance to bacteria. In this study, we found that the outer membrane protein OmpW was related to salt stress in Vibrio cholerae and that ompW gene transcription and expression were up-regulated in cultures containing high NaCl concentrations. Deletion of ompW resulted in reduced V. cholerae growth in hypersaline culture conditions. Supplements of the compatible solutes betaine, L-carnitine, or L-lysine enhanced the growth of V. cholerae in hypersaline media. Supplements of betaine or L-lysine had the same growth enhancement effect on the ompW-deletion mutant cultured in hypersaline media, whereas L-carnitine supplementation did not restore mutant growth. In addition, the uptake of L-carnitine was decreased in the ompW-deletion mutant. Our study showed that among the multiplex factors that enhance the hypersaline tolerance of V. cholerae, OmpW also plays a role by transporting L-carnitine.