Opioid receptor‐like (ORL1) receptor distribution in the rat central nervous system: Comparison of ORL1 receptor mRNA expression with 125I‐[14Tyr]‐orphanin FQ binding
Opioid receptor‐like (ORL1) receptor distribution in the rat central nervous system: Comparison of ORL1 receptor mRNA expression with 125I‐[14Tyr]‐orphanin FQ binding
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DOI：
10.1002/(sici)1096-9861(19991004)412:4
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发表时间：
1999-10
影响因子：
2.5
通讯作者：
C. Neal;A. Mansour;Rainer R. Reinscheid;H. Nothacker;O. Civelli;H. Akil;S. Watson
中科院分区：
文献类型：
--
作者：
C. Neal;A. Mansour;Rainer R. Reinscheid;H. Nothacker;O. Civelli;H. Akil;S. Watson
最近发现的神经肽孤啡肽(OFQ)及其阿片受体样受体(ORL1)的结构特征提示μ，κ和δ阿片系统。近年来，有关OFQ免疫反应和mRNA表达的解剖分布已有报道。在目前的分析中，我们比较了在成年大鼠中枢神经系统(CNS)中孤儿受体mRNA的表达与孤儿FQ结合在ORL1受体上的分布。以~(125)I-[~(14)Tyr]-OFQ为放射性配基，采用体外受体放射自显影技术，分析孤啡肽受体在大鼠中枢神经系统的结合情况。孤啡肽的结合部位主要集中在嗅前核、外侧隔核、前脑腹侧核、下丘脑多个核团、海马结构、杏仁基底外侧核和内侧核、中央灰质、桥核、脚间核、黑质、中缝复合体、蓝斑、前庭核复合体和脊髓。原位杂交结果显示，ORL1mRNA表达阳性细胞主要分布于多个皮质区域、嗅前核、外侧隔核、梨状内核、前脑腹侧、下丘脑多个核团、嗅束外侧核、杏仁内侧核、海马结构、黑质、腹侧被盖区、中央灰质、中缝复合体、蓝斑、多个脑干运动核团、下橄榄核、小脑深部核、前庭核复合体、孤束核、网状结构、背根神经节和脊髓。ORL1mRNA和结合的弥漫性分布支持孤儿FQ在多种中枢功能中发挥广泛的作用，包括运动和平衡控制、强化和奖励、伤害性感受、应激反应、性行为、攻击和生理过程的自主控制。J.Comp.神经。412：563-605,1999。©1999 Wiley-Liss，Inc.
The recently discovered neuropeptide orphanin FQ (OFQ), and its opioid receptor‐like (ORL1) receptor, exhibit structural features suggestive of the μ, κ, and δ opioid systems. The anatomic distribution of OFQ immunoreactivity and mRNA expression has been reported recently. In the present analysis, we compare the distribution of orphanin receptor mRNA expression with that of orphanin FQ binding at the ORL1 receptor in the adult rat central nervous system (CNS). By using in vitro receptor autoradiography with 125I‐[14Tyr]‐OFQ as the radioligand, orphanin receptor binding was analyzed throughout the rat CNS. Orphanin binding sites were densest in several cortical regions, the anterior olfactory nucleus, lateral septum, ventral forebrain, several hypothalamic nuclei, hippocampal formation, basolateral and medial amygdala, central gray, pontine nuclei, interpeduncular nucleus, substantia nigra, raphe complex, locus coeruleus, vestibular nuclear complex, and the spinal cord. By using in situ hybridization, cells expressing ORL1 mRNA were most numerous throughout multiple cortical regions, the anterior olfactory nucleus, lateral septum, endopiriform nucleus, ventral forebrain, multiple hypothalamic nuclei, nucleus of the lateral olfactory tract, medial amygdala, hippocampal formation, substantia nigra, ventral tegmental area, central gray, raphe complex, locus coeruleus, multiple brainstem motor nuclei, inferior olive, deep cerebellar nuclei, vestibular nuclear complex, nucleus of the solitary tract, reticular formation, dorsal root ganglia, and spinal cord. The diffuse distribution of ORL1 mRNA and binding supports an extensive role for orphanin FQ in a multitude of CNS functions, including motor and balance control, reinforcement and reward, nociception, the stress response, sexual behavior, aggression, and autonomic control of physiologic processes. J. Comp. Neurol. 412:563–605, 1999. © 1999 Wiley‐Liss, Inc.