Exfoliation of gastric pit-parietal cells into the gastric lumen associated with a stimulation of isolated rat gastric mucosa in vitro: a morphological study by the application of cryotechniques
Exfoliation of gastric pit-parietal cells into the gastric lumen associated with a stimulation of isolated rat gastric mucosa in vitro: a morphological study by the application of cryotechniques
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DOI：
10.1007/s00418-008-0403-1
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发表时间：
2008-06-01
影响因子：
2.3
通讯作者：
Suganuma, Tatsuo
中科院分区：
文献类型：
--
作者：
Aoyama, Fumiyo;Sawaguchi, Akira;Suganuma, Tatsuo
阐明维持正常胃上皮的细胞动力学具有重要的临床病理学意义。在刺激后分离的大鼠胃粘膜中，许多细胞脱落到胃凹区的胃腔中。本研究旨在利用冷冻技术的优势来阐明脱落细胞的起源及其组织化学特征。结果，大多数脱落细胞被鉴定为用花生凝集素和抗H+/K+-ATP酶抗体标记的凹坑壁细胞。定量分析验证了刺激后分离的胃粘膜中脱落细胞数量随时间的增加。脱落的细胞表现出弥漫性细胞内 E-钙粘蛋白染色，表明粘附分子在细胞脱落之前解离。值得注意的是，大多数脱落细胞的凋亡标记物（TUNEL 染色和 caspase-3）呈阴性。超微结构上，脱落的凹壁细胞中经常可见由 H+/K+-ATPase 阳性膜组成的自噬体样结构。此外，凹陷壁细胞的脱落伴随着其基底部分与邻近表面粘液细胞的细胞质突起的密封。目前的形态学研究结果为体外胃上皮细胞动力学提供了新的见解。
It is clinicopathologically important to elucidate the cell kinetics for the maintenance of normal gastric epithelium. In a rat gastric mucosa isolated after stimulation, a number of cells were exfoliated into the gastric lumen of the pit region. The present study was undertaken to clarify the origin of exfoliated cells and their histochemical profiles by taking the advantages of cryotechniques. As results, most of the exfoliated cells were identified as pit-parietal cells labeled with both peanut-lectin and anti-H+/K+-ATPase antibody. Quantitative analysis verified a time-dependent increase in the number of exfoliated cells in the gastric mucosa isolated after stimulation. The exfoliated cells exhibited a diffuse intracellular staining for E-cadherin, suggesting a dissociation of the adhesion molecule prior to the cell exfoliation. It should be noted that most of the exfoliated cells were negative to the apoptotic markers (TUNEL staining and caspase-3). Ultrastructurally, autophagosome-like structures consisting of H+/K+-ATPase positive membranes were frequently seen in the exfoliated pit-parietal cells. In addition, the pit-parietal cell exfoliation was accompanied by sealing of their basal portion with the cytoplasmic processes of adjacent surface mucous cells. The present morphological findings provide a new insight into the cell kinetics in the gastric epithelium in vitro.