A new model for partial immobilization of rat hind limb after Achilles tendon excision/reinterposition
A new model for partial immobilization of rat hind limb after Achilles tendon excision/reinterposition
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DOI：
10.3906/vet-1206-8
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发表时间：
2013-01-01
影响因子：
0.6
通讯作者：
Demirutku, Alper
中科院分区：
文献类型：
--
作者：
Gungormus, Cansin;Cetinkaya, Mehmet Alper;Demirutku, Alper
已经有几次尝试通过手术或用移植材料替换来修复跟腱。由于组织修复是一个复杂的过程，肌腱愈合缓慢。对于大鼠来说，肢体的固定在恢复过程中至关重要。有固定方法，如石膏或纤维铸造。根据长期恢复，大多数手术会导致皮肤溃疡，体重下降，检查手术区域的限制，以及从石膏中滑出。我们的目的是在缺损周围缝合固定。解剖30只雄性Wistar大鼠（250 +/-50 g，8 +/-2周龄）的肌腱，立即用8-0不可吸收缝线近端-远端缝合切断端。为了部分固定，将带有4-0缝线的针在腓肠肌和跟骨之间向近端穿过3 mm，向远端穿过3 mm。在跟骨附近打了一个结。用连续的福特联锁6-0缝合模式闭合皮肤。在2周和4周后处死动物。与对侧完整肌肉相比，未观察到肌肉萎缩的平均值存在差异。未观察到溃疡、疼痛或肿胀。肌腱完整，显示出优良的上级组织学特征。最近的技术似乎是有效的应用力量，以保护从腓肠肌的肌肉拉伸应力在恢复期间的缺损区。
There have been several attempts to repair the Achilles tendon by surgery or replacement with graft materials. Because tissue repair is a complex process, tendon healing occurs slowly. In rats, immobilization of the limb is essential during recovery. There are immobilization methods such as plaster or fiber casting. According to prolonged recovery, most of the procedures cause skin ulceration, decrease in weight, restrictions in checking the surgery area, and slipping out of the cast. Our aim was to apply a suture around the defect for immobilization. Tendons of 30 male Wistar rats (250 +/- 50 g and aged 8 +/- 2 weeks) were dissected and the cut ends were immediately sutured proximally-distally with an 8-0 nonabsorbable suture. For partial immobilization a needle with a 4-0 suture was passed 3 mm proximally and 3 mm distally between the gastrocnemius and calcaneus. A knot was applied close to the calcaneus. The skin was closed with a continuous, Ford interlocking 6-0 suture pattern. The animals were sacrificed after 2 and 4 weeks. No difference was observed in means of muscle atrophy when compared to contralateral intact muscle. No ulcer, sore, or swelling was observed. Tendons were intact and showed superior histological characteristics. Recent techniques seem to be effective in applying strength to protect the defect area from muscle tensile stress of the gastrocnemius during the recovery period.