Consumption of Whey in Combination with Dairy Medium-Chain Fatty Acids (MCFAs) may Reduce Lipid Storage due to Urinary Loss of Tricarboxylic Acid Cycle Intermediates and Increased Rates of MCFAs Oxidation
Consumption of Whey in Combination with Dairy Medium-Chain Fatty Acids (MCFAs) may Reduce Lipid Storage due to Urinary Loss of Tricarboxylic Acid Cycle Intermediates and Increased Rates of MCFAs Oxidation
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DOI：
10.1002/mnfr.201601048
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发表时间：
2017-12-01
影响因子：
5.2
通讯作者：
Dalsgaard, Trine Kastrup
中科院分区：
文献类型：
--
作者：
Amer, Bashar;Clausen, Morten Rahr;Dalsgaard, Trine Kastrup
范围：本文的目的是研究代谢组的变化是否可以解释超重成年人在食用含有高水平中链脂肪酸（MCFAs）和酪蛋白或乳清的黄油后观察到的身体组成的变化。方法与结果：采用GC-TOF和LC-Q/MS对52名腹部超重成人进行为期12周的随机双盲干预，分析其血浆和尿液中的代谢物。参与者每天消耗63克乳脂（MCFAs含量高低）和60克蛋白质（乳清或酪蛋白）。在乳清+高mcfas组中观察到尿中三羧酸循环代谢物的丢失和血液中甘油的增加，表明通过排出底物可能降低合成代谢过程，如脂肪生成。大量摄入MCFAs导致尿己二酸（与蛋白质类型无关）和血浆己二酸（含乳清）水平升高，表明MCFAs氧化可能增加，这可能导致能量损失。结论：蛋白质类型对整体代谢谱的影响最大，但肝脏中MCFAs的ω -氧化似乎是摄入高MCFAs后观察到的体脂量减少的主要原因，与蛋白质类型无关。
Scope: The aim of the paper is to investigate whether changes in the metabolome could explain observed changes in body composition in overweight adults after consumption of butter with high level of medium-chain fatty acids (MCFAs) in combination with casein or whey.Methods and results: With GC-TOF and LC-Q/MS, metabolites in plasma and urine from a 12-week randomized double-blinded human intervention including 52-abdominally overweight adults were analyzed. The participants consumed 63 g per day of milk fat (high or low in MCFAs) and 60 g per day of protein (whey or casein). Urinary loss of the tricarboxylic acid cycle metabolites and a concomitantly increase of glycerol in blood were observed in the whey + high-MCFAs group, indicating potential lower anabolic processes, such as lipogenesis, by draining substrates. High intake of MCFAs resulted in elevated level of urinary adipic (independently of protein type) and plasma sebacic acid (with whey), indicating a potential increase in oxidation of MCFAs, which might lead to energy loss.Conclusion: The type of protein showed highest effect on the overall metabolic profiles, but omega-oxidation of MCFAs in the liver seemed to be the main reason for the observed reduction in body fat mass after consumption of high MCFAs, independent of type of protein.