Evaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Blood Isolates of Vibrio Species
Evaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Blood Isolates of Vibrio Species
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DOI：
10.1128/jcm.00105-15
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发表时间：
2015-05-01
影响因子：
9.4
通讯作者：
Hsueh, Po-Ren
中科院分区：
文献类型：
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作者：
Cheng, Wern-Cherng;Jan, I-Shiow;Hsueh, Po-Ren
在通过16 S rRNA和rpoB基因测序分析鉴定的56株弧菌属血液分离株中，（MALDI-TOF MS）系统正确鉴定了创伤弧菌（n = 20）、副溶血性弧菌（n = 2）、而非O 1/O 139群霍乱弧菌（33株）均未达到种水平。使用新创建的MALDI-TOF MS数据库正确鉴定了所有这些血清群非O 1/O 139霍乱弧菌分离株。
Among 56 blood isolates of Vibrio species identified by sequencing analysis of 16S rRNA and rpoB genes, the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system correctly identified all isolates of Vibrio vulnificus (n = 20), V. parahaemolyticus (n = 2), and V. fluvialis (n = 1) but none of the isolates of serogroup non-O1/O139 (non-serogroup O1, non-O139) V. cholerae (n = 33) to the species level. All of these serogroup non-O1/O139 V. cholerae isolates were correctly identified using the newly created MALDI-TOF MS database.