Serum levels of β-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma
Serum levels of β-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma
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DOI：
10.3892/or.2011.1355
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发表时间：
2011-10-01
期刊：
影响因子：
4.2
通讯作者：
Daoud, Sayed S.
中科院分区：
文献类型：
--
作者：
Zekri, Abdel-Rahman N.;Bahnassy, Abeer A.;Daoud, Sayed S.
随着丙型肝炎病毒(HCV)感染率的增加，全球肝细胞癌(HCC)的发病率也在不断上升，这引发了人们对寻找更多的肝细胞癌血清标志物的兴趣。在这项研究中，我们探讨了血清E-钙粘素、ICAM、基质金属蛋白酶-2、血管内皮生长因子、骨桥蛋白和β-连环蛋白作为潜在的丙型肝炎病毒/基因4相关肝癌诊断标志物的临床应用。健康体检者20例，无症状丙型肝炎病毒携带者(ASC)11例，慢性肝炎(CH)患者28例，肝癌患者32例。采用双抗体夹心酶联免疫吸附试验(ELISA)测定血清蛋白质水平。应用受试者-操作特征(ROC)曲线分析，报告曲线下面积(AUC)及其95%可信区间(CI)，评估每个候选标志物的诊断准确性。我们发现，与CH、ASC和健康对照组相比，肝细胞癌患者的血清β-连环蛋白水平显著升高。在所研究的6种标志物中，β-连环蛋白也是唯一能显著区分肝细胞癌和慢性肝炎患者的标志物，因此，β-连环蛋白可被认为是早期诊断感染丙型肝炎病毒4型的患者中与丙型肝炎相关的肝细胞癌的潜在标记物。
The global rising incidence of hepatocellular carcinoma (HCC), which parallels the increase of hepatitis C virus (HCV) prevalence, has sparked a renewed interest in discovering additional HCC serum markers. In this study, we investigated the clinical use of serum E-cadherin, ICAM, MMP-2, VEGF, OPN and beta-catenin as potential diagnostic makers for HCV/genotype 4-associated HCC. Twenty cases of healthy subjects, 11 cases with asymptomatic HCV/genotype 4 carriers (ASC), 28 chronic hepatitis (CH) cases and 32 patients with HCC were enrolled in this study. Serum levels of proteins were measured by a sandwich-enzyme-linked (ELISA) assay. The diagnostic accuracy of each candidate marker was evaluated using receiver-operating characteristic (ROC) curve analysis, reporting the area under the curve (AUC) and its 95% confidence interval (CI). We demonstrated that serum beta-catenin levels were significantly elevated in patients with HCC compared to those with CH, ASC and healthy controls. Among the six studied markers, beta-catenin was also found to be the only marker that can significantly discriminate between patients with HCC and those with CH; therefore, beta-catenin could be considered as a potential marker for early diagnosis of HCV-associated HCC in patients infected with HCV genotype 4.