The Prevalence of Antibodies to Human Herpesvirus 8 and Hepatitis B virus in Patients in Two Hospitals in Tanzania
The Prevalence of Antibodies to Human Herpesvirus 8 and Hepatitis B virus in Patients in Two Hospitals in Tanzania
复制标题
DOI：
10.1002/jmv.21852
复制
发表时间：
2010-09-01
影响因子：
12.7
通讯作者：
Ippolito, G.
中科院分区：
文献类型：
--
作者：
Meschi, S.;Schepisi, M. Sane;Ippolito, G.
本研究的目的是确定坦桑尼亚联合共和国两个地区发热患者中人类疱疹病毒8 （HHV-8）的血清阳性率和乙型肝炎病毒（HBV）感染的免疫状况。2007年2月至3月期间，在奔巴岛和托萨马甘加从336名门诊病人身上采集了血液样本，并送到（意大利）罗马的病毒学实验室进行检测。采用两种内部分子方法扩增HHV-8 DNA和HBV-DNA，采用免疫荧光法（IFA）检测抗HHV-8抗体滴度，采用微孔板酶免疫法（MEIA）检测抗hcv、HBsAg、抗hbs和抗hbc。HHV-8血清阳性率为30.7%（96/313）。奔巴岛的患病率低于托萨马甘加岛（14.4%对46.3%）。低滴度的HHV-8 IgG (
The aim of this study was to determine the seroprevalence of human herpesvirus 8 (HHV-8) and the immunization status for hepatitis B virus (HBV) infection in febrile patients in two districts of the United Republic of Tanzania. Between February and March 2007, blood samples were collected in Pemba Island and Tosamaganga from 336 outpatients and sent to the Virology Laboratory in Rome (Italy) for testing. HHV-8 DNA and HBV-DNA were amplified by two in-house molecular methods, anti-HHV-8 antibody titers were determined by an immunofluorescence assay (IFA), and anti-HCV, HBsAg, anti-HBs, and anti-HBc were evaluated by microplate enzyme immunoassay (MEIA). The seroprevalence of HHV-8 was 30.7% (96/313). In Pemba Island, the prevalence was lower than in Tosamaganga (14.4% vs. 46.3%). A higher prevalence of low titers of HHV-8 IgG (