Murine T cell-derived contractile activity for human bronchial smooth muscle cells
Murine T cell-derived contractile activity for human bronchial smooth muscle cells
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鼠 T 细胞衍生的人支气管平滑肌细胞收缩活性
DOI：
10.1159/000337743
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Abe;A.;Koyama;S.;Ohtomo;T.;Kitamura;N.;Kaminuma;O.;and Mori;A.
吸入性转移辅助性T(Th)细胞可在抗原攻击时产生迟发性哮喘反应。方法用固定化抗CD3抗体刺激小鼠Th克隆，观察其对小鼠支气管平滑肌(BSM)的收缩作用。结果激活但不静息的小鼠Th克隆的培养上清可引起晚期哮喘反应，诱导含有人BSM细胞的胶原凝胶收缩。结论活化的Th细胞在体外对BSM具有收缩活性。
BackgroundAdoptive transfer of helper T (Th) cells conferred a late asthmatic response upon antigen challenge. A possible production of a contractile activity for bronchial smooth muscle (BSM) by activated Th cells was examined.MethodMurine Th clones were stimulated with immobilized anti-CD3 antibody in the presence or absence of anti-CD28 antibody. Culture supernatants were dialyzed and then applied to the collagen gels containing cultured human BSM cells.ResultsCulture supernatants of activated but not resting murine Th clones that conferred a late asthmatic response, induced the contraction of human BSM cell-containing collagen gels.ConclusionActivated Th cells produce a contractile activity for BSM in vitro.