Chicken volatiles repel host-seeking malaria mosquitoes.
Chicken volatiles repel host-seeking malaria mosquitoes.
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DOI：
10.1186/s12936-016-1386-3
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发表时间：
2016-07-21
期刊：
影响因子：
3
通讯作者：
Ignell R
中科院分区：
文献类型：
--
作者：
Jaleta KT;Hill SR;Birgersson G;Tekie H;Ignell R
阿拉伯按蚊是撒哈拉以南非洲疟疾的主要媒介，在室内和室外以人类和其他脊椎动物宿主为食，使其成为一个难以用现有控制方法控制的物种。因此，需要减少人与病媒相互作用的新方法，以提高病媒控制方案的影响。调查寄主歧视过程背后的机制。阿拉伯斑潜蝇可以为开发新的防治技术提供有价值的知识。本研究通过寄主普查和血餐分析来确定按蚊的寄主选择行为。阿拉伯。由于蚊子主要利用嗅觉在寄主之间进行选择和区分，因此收集了首选的非人类寄主和非寄主物种的挥发性顶空。采用气相色谱和电触角检测相结合的分析方法，再用气相色谱和质谱仪相结合的方法，鉴定了顶空样品中的活性成分。在野外条件下测试了已鉴定的非寄主化合物对寻找寄主的疟疾蚊子的驱避效果。宿主普查和血餐分析表明，An.阿拉比斯在室内寻找寄主时强烈喜欢人血，而在室外发现时则随机捕食牛、山羊和绵羊。然而，一个。尽管鸡的丰度相对较高，但阿拉比斯人不喜欢鸡，这表明鸡是这种媒介的非宿主物种。在非寄主物种的头腔中发现了11种生物活性化合物。其中6个是物种特有的，其中4个是用气相色谱和质谱仪相结合的方法鉴定出来的。在田间测试时，鸡专用化合物丁酸异丁酯、萘、十六烷和反式柠檬烯氧化物，以及通用宿主化合物柠檬烯、顺式柠檬烯氧化物和β-月桂烯，与阴性对照相比，显著减少了室内诱捕量。当把笼子里的鸡悬挂在诱捕器旁边时，诱捕量也显著减少。非寄主挥发物排斥寻找寄主的AN。因此在寄主歧视中起着重要的作用。因此，这项研究表明，非寄主挥发物可以与既定的控制方案相结合，为有蚊媒传播疾病风险的人类提供保护。本文的在线版本(doi：10.1186/s12936-0161386-3)包含补充材料，授权用户可以使用。
Anopheles arabiensis is a dominant vector of malaria in sub-Saharan Africa, which feeds indoors and outdoors on human and other vertebrate hosts, making it a difficult species to control with existing control methods. Novel methods that reduce human-vector interactions are, therefore, required to improve the impact of vector control programmes. Investigating the mechanisms underlying the host discrimination process in An. arabiensis could provide valuable knowledge leading to the development of novel control technologies. In this study, a host census and blood meal analysis were conducted to determine the host selection behaviour of An. arabiensis. Since mosquitoes select and discriminate among hosts primarily using olfaction, the volatile headspace of the preferred non-human host and non-host species, were collected. Using combined gas chromatography and electroantennographic detection analysis followed by combined gas chromatography and mass spectrometry, the bioactive compounds in the headspace collections were identified. The efficiency of the identified non-host compounds to repel host-seeking malaria mosquitoes was tested under field conditions. The host census and blood meal analyses demonstrated that An. arabiensis strongly prefers human blood when host seeking indoors, while it randomly feeds on cattle, goats and sheep when found outdoors. However, An. arabiensis avoids chickens despite their relatively high abundance, indicating that chickens are a non-host species for this vector. Eleven bioactive compounds were found in the headspace of the non-host species. Six of these were species-specific, out of which four were identified using combined gas chromatography and mass spectrometry. When tested in the field, the chicken-specific compounds, isobutyl butyrate, naphthalene, hexadecane and trans-limonene oxide, and the generic host compounds, limonene, cis-limonene oxide and β-myrcene, significantly reduced trap catches within the house compared to a negative control. A significant reduction in trap catch was also observed when suspending a caged chicken next to the trap. Non-host volatiles repel host-seeking An. arabiensis and thus play a significant role in host discrimination. As such, this study demonstrates that non-host volatiles can provide protection to humans at risk of mosquito-vectored diseases in combination with established control programmes. The online version of this article (doi:10.1186/s12936-016-1386-3) contains supplementary material, which is available to authorized users.