Studies of the ethanol-induced interdigitated gel phase in phosphatidylcholines using the fluorophore 1,6-diphenyl-1,3,5-hexatriene.
Studies of the ethanol-induced interdigitated gel phase in phosphatidylcholines using the fluorophore 1,6-diphenyl-1,3,5-hexatriene.
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使用荧光团 1,6-二苯基-1,3,5-己三烯研究乙醇诱导的磷脂酰胆碱交错凝胶相。
DOI：
10.1021/bi00426a015
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
McIntosh,TJ
中科院分区：
文献类型：
--
作者：
Nambi,P;Rowe,ES;McIntosh,TJ
1988年7月18日收到的修订版摘要：现在已经确定，一些两亲性分子（如乙醇）可以诱导磷脂酰胆碱形成完全交叉的凝胶相。我们先前已经表明，醇类如乙醇诱导磷脂酰胆碱的两相熔融行为[Rowe，E. S.（1983）Biochemistry 22，3299-3305]，但在磷脂酰乙醇胺中不存在[Rowe，E. S.（1985）Biochim. Biophys. Acta 813，321-330]。Simonand McIntosh [（1984）Biochim. Biophys. Acta 773，169-172]表明磷脂酰胆碱中醇诱导的两相熔融行为是酰基链交错的结果。在本研究中，我们证明了在乙醇存在下，使用常用的荧光团1，6-二苯基-1，3，5-己三烯（DPH）的荧光性质检测DPPC和DSPC向叉指相的转变。通过关联荧光和X射线衍射结果，我们证明了使用荧光来研究从非叉指相到叉指相的相变。使用这种方法，我们已经研究了温度和乙醇浓度依赖性的诱导的交叉相DSPC和DPPC和表明，由乙醇诱导的交叉是温度依赖性的，与较高的温度有利于交叉。
Revised Manuscript Received July 18, 1988 abstract: It is now well established that a number of amphiphilic molecules such as ethanol can induce the formation of the fully interdigitated gelphase in phosphatidylcholines. We have shown earlier that alcohols such as ethanol induce biphasic melting behavior in phosphatidylcholines [Rowe, E. S.(1983) Biochemistry 22, 3299-3305] but not in phosphatidylethanolamines [Rowe, E. S.(1985) Biochim. Biophys. Acta 813, 321-330]. Simonand McIntosh [(1984) Biochim. Biophys. Acta 773, 169-172] showed that the alcohol-induced biphasic melting behavior in phosphatidylcholines is a consequence of acyl chain interdigitation. In the present study we demonstrate the detection of the transition of DPPC and DSPC to the interdigitated phase in thepresence of ethanol using the fluorescence properties of the commonly used fluorophore 1, 6-diphenyl-1, 3, 5-hexatriene (DPH). By correlating fluorescence and X-ray diffraction results, we have demonstrated the use of fluorescence to study the phase transition from the noninterdigitated to the in-terdigitated phase. Using this method, we have investigated the temperature and ethanol concentration dependence of the induction of the interdigitated phase in DSPCand DPPC and shown that the induction of interdigitation by ethanol is temperature dependent, with higher temperature favoring interdigitation.