Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System
Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System
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DOI：
10.1007/978-1-61737-957-4_14
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发表时间：
2011-01-01
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影响因子：
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通讯作者：
Endo, Takashi R.
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文献类型：
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作者：
Endo, Takashi R.
小麦族物种的基因组庞大而复杂，这给获得它们的全序列数据带来了困难。一些被称为杀配子(GC)染色体的外源染色体被导入普通小麦，会导致染色体断裂，导致缺失和易位的产生。这些诱导缺失已被建立为普通小麦的缺失库。该CC系统也能有效地诱导普通小麦染色体的断裂。通过染色体显带和荧光原位杂交可以鉴定诱发的异常染色体，并可在普通小麦中建立分离系。该Gs系统可用于对小麦属植物的单染色体进行解剖。
Triticeae species have a large and complex genome, which has made it difficult to obtain their-sequence data. Some alien chromosomes called the gametocidal (Gc) chromosomes introduced into common wheat can induce chromosomal breakage resulting in the generation of deletions and translocations. The induced deletions have been established as deletion stocks in common wheat. This Cc system is also effective in inducing chromosomal breakages in Triticeae chromosomes added to common wheat. The induced aberrant chromosomes can be identified by chromosome banding and fluorescence in situ hybridization and can be established in common wheat as dissection lines. This Gs system will be useful to dissect the single chromosomes of Triticeae species.