Raman spectroscopy of endoscopic colonic biopsies from patients with ulcerative colitis to identify mucosal inflammation and healing.
Raman spectroscopy of endoscopic colonic biopsies from patients with ulcerative colitis to identify mucosal inflammation and healing.
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DOI：
10.1364/boe.7.002022
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发表时间：
2016-05-01
影响因子：
3.4
通讯作者：
Subramanian V
中科院分区：
文献类型：
--
作者：
Addis J;Mohammed N;Rotimi O;Magee D;Jha A;Subramanian V
拉曼光谱用于区分溃疡性结肠炎患者的粘膜愈合（或静止）和炎症结肠组织，如内窥镜评估。从60个活检组织样品的拉曼光谱的分析中，在静止组织和发炎组织的光谱之间识别出明显的差异。三个类胡萝卜素峰被发现是大约两倍的强度在发炎的组织。发现两个磷脂峰在发炎组织中显著较低。使用多元统计分析，我们表明，这五个峰值可以用来区分内窥镜静止和发炎组织。我们还将拉曼数据与组织的组织学评估相关联。发现五个峰中的四个在组织学愈合（或静止）和组织学发炎组织的光谱之间显著不同。这些发现表明拉曼光谱能够准确地将结肠组织分类为静止或发炎，而不管是否遵循内窥镜或组织学分级方案。因此，我们表明，拉曼光谱可能被用作早期诊断工具，用于评估溃疡性结肠炎患者的粘膜愈合或炎症的存在。
Raman spectroscopy was used to differentiate between mucosally healed (or quiescent) and inflamed colon tissue, as assessed endoscopically, in patients with ulcerative colitis. From the analysis of the Raman spectra of 60 biopsy tissue samples, clear differences were identified between the spectra of the quiescent and inflamed tissue. Three carotenoid peaks were found to be approximately twice as intense in the inflamed tissue. Two phospholipid peaks were found to be significantly lower in the inflamed tissue. Using multivariate statistical analysis, we show that these five peaks can be used to discriminate between endoscopically quiescent and inflamed tissue. We also correlated the Raman data with a histological assessment of the tissue. Four of the five peaks were found to be significantly different between the spectra of histologically healed (or quiescent) and histologically inflamed tissue. These findings indicate the ability of Raman spectroscopy to accurately classify colon tissue as either quiescent or inflamed, irrespective of whether an endoscopic or histological grading scheme is followed. We thus demonstrate that Raman spectroscopy could potentially be used as an early diagnosis tool for assessing the presence of mucosal healing or inflammation in patients with ulcerative colitis.