Microscopic tomography by laser scanning microscopy and its three‐dimensional reconstruction
Microscopic tomography by laser scanning microscopy and its three‐dimensional reconstruction
复制标题
激光扫描显微断层扫描及其三维重建
作者：
T. Takamatsu;S. Fujita
我们研制了一种新型的共聚焦激光扫描显微镜，它配备了两个振镜，可以摆动激光光束。有了这个装置，我们就可以观察到大而脆弱的标本。用聚焦的激光束作为光源，减小耀斑和探测器前的针孔，消除散焦数据，可以得到深度分辨的荧光图像。本系统的扫描装置可以消除机械振动，扫描范围广，以获得低放大倍数的图像。用光学方法可以从原位厚样品获得高分辨率、高对比度的薄层图像。我们把这项技术称为“显微断层成像”。将激光扫描显微镜与彩色图像分析仪相结合，半自动生成生物材料的三维图像，并提供其内部信息。
We have developed a new confocal laser scanning microscope equipped with two galvanometer mirrors which swing the laser beam. With this set up we can observe large and fragile specimens. Using a focused laser beam as light source to minimize ‘flare’ and a pinhole in front of a photodetector to eliminate out‐of‐focus data, we could obtain a depth‐discriminated fluorescence image. The scanning apparatus of our system can eliminate mechanical vibration and sweep widely, to obtain images at a low magnification. A thinly sectioned image with high resolution and high contrast could be obtained optically from an in situ thick specimen. We have called this technique ‘microscopic tomography’. Combining the laser scanning microscope with the colour image analyser generated semi‐automatically a three‐dimensional picture of the biological material with information on its interior.