GENITAL HUMAN PAPILLOMAVIRUS INFECTION IN FEMALE UNIVERSITY-STUDENTS AS DETERMINED BY A PCR-BASED METHOD
GENITAL HUMAN PAPILLOMAVIRUS INFECTION IN FEMALE UNIVERSITY-STUDENTS AS DETERMINED BY A PCR-BASED METHOD
复制标题
DOI：
10.1001/jama.265.4.472
复制
发表时间：
1991-01-23
影响因子：
120.7
通讯作者：
MANOS, MM
中科院分区：
文献类型：
--
作者：
BAUER, HM;TING, Y;MANOS, MM
采用两种方法（美国食品和药物管理局批准的ViraPap检测和聚合酶链反应（PCR） DNA扩增技术）在宫颈和外阴部位检测生殖器人类乳头瘤病毒（HPV）的存在，467名妇女到一所大学健康服务中心进行常规年度妇科检查。PCR系统提供了敏感的检测广泛的生殖器HPV类型。使用PCR，我们发现46%的研究人群感染了HPV；ViraPap测试显示，受感染的比例为11%。聚合酶链反应分析表明，69%的hpv阳性妇女在两个生殖器部位都受到感染。随后的HPV型别测定显示，33%的研究人群有HPV型6、11、16、18、31、33、35、39、45、51、52或其他先前分离的型别，13%的人有尚未确定的型别。几乎所有（92%）经巴氏涂片诊断为尖锐湿疣异型或不典型增生的妇女（n = 12）都是HPV阳性。PCR方法被证明是一种在大量临床样本中检测HPV的信息丰富和快速的方法。我们的结果表明，生殖器HPV感染是常见的性活跃的年轻女性。
The presence of genital human papillomavirus (HPV) was determined at cervical and vulvar sites using two methods, the Food and Drug Administration-approved ViraPap test and polymerase chain reaction (PCR) DNA amplification technology, in 467 women presenting to a university health service for a routine annual gynecologic examination. The PCR system afforded the sensitive detection of a broad spectrum of genital HPV types. Using PCR, we found that 46% of the study population was infected with HPV; the ViraPap test showed a prevalence of 11% infected. PCR analyses demonstrated that 69% of the HPV-positive women were infected at both genital sites. Subsequent HPV-type determination showed that 33% of the study population had HPV types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, or other previously isolated types, and 13% had yet unidentified types. Almost all (92%) of the women diagnosed by Papanicolaou smear with condylomatous atypia or dysplasia (n = 12) were HPV positive. The PCR method proved to be an informative and rapid way to detect HPV in large numbers of clinical samples. Our results demonstrate that genital HPV infection is common among sexually active young women.