HAFNIA-ALVEI, A PROBABLE CAUSE OF DIARRHEA IN HUMANS
HAFNIA-ALVEI, A PROBABLE CAUSE OF DIARRHEA IN HUMANS
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DOI：
10.1128/iai.59.4.1507-1513.1991
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发表时间：
1991-04-01
影响因子：
3.1
通讯作者：
TZIPORI, S
中科院分区：
文献类型：
--
作者：
ALBERT, MJ;ALAM, K;TZIPORI, S
蜂房哈夫尼菌是肠杆菌科的一种，是从一名9个月大的儿童粪便中培养出的唯一一种细菌，该儿童因3天的水样腹泻而入院。分离的H. alvei不能产生热不稳定或热稳定的肠毒素或志贺样毒素I或II，并且不侵入HeLa细胞，也不引起豚鼠眼中的角膜结膜炎（通过Sereny试验确定）。然而，该菌株在12只具有可拆卸肠扎带的成年家兔中的8只（可拆卸肠扎带-成年家兔腹泻[RITARD]试验）和2只经口喂养动物中的1只中诱导腹泻。在8只RITARD试验家兔和3只经口喂养家兔中，大肠杆菌K-12均未诱导腹泻。受累动物的显微镜检查显示肠粘膜中度炎性细胞浸润，其中回肠和结肠中的细菌附着于表面上皮和微绒毛边缘丢失明显。电子显微镜显示顶端表面的细胞修饰，杯状或基座形成，并在细菌“附着-消失”部位增加末端网密度，这是肠致病性大肠杆菌腹泻的众所周知的特征和机制。杆菌H. alvei，排除了自然发生的兔肠致病性E.大肠杆菌菌株，这是已知的产生类似的病变。结论至少有部分H. alvei具有引起腹泻的潜力，并且附着-消失是除E.杆菌
Hafnia alvei, a member of the family Enterobacteriaceae, was the only species of bacteria cultured from the stool of a 9-month-old child who was admitted with a 3-day history of watery diarrhea. The isolated strain of H. alvei failed to produce heat-labile or heat-stable enterotoxins or Shiga-like toxin I or II and did not invade HeLa cells, nor did it cause keratoconjunctivitis (determined by the Sereny test) in a guinea pig's eye. The strain, however, induced diarrhea in 8 of 12 adult rabbits with removable intestinal ties (removable intestinal tie-adult rabbit diarrhea [RITARD] assay) and in 1 of 2 orally fed animals. No diarrhea could be induced with Escherichia coli K-12 in eight RITARD assay rabbits and three orally fed rabbits, respectively. Microscopic examination of affected animals revealed moderate inflammatory cellular infiltration of the intestinal mucosa, in which bacterial attachment to the surface epithelium and loss of the microvillus border were evident in the ileum and colon. Electron microscopy demonstrated cellular modifications of the apical surface, with cupping or pedestal formation and increased terminal web density at sites of bacterial "attachment-effacement," a well-known characteristic and mechanism of diarrhea of enteropathogenic E. coli. Identical lesions were also induced by H. alvei in rabbit ileal loops, which ruled out naturally occurring rabbit enteropathogenic E. coli strains, which are known to produce similar lesions. It is concluded that at least some strains of H. alvei have the potential to cause diarrhea and that attachment-effacement is a virulence characteristic shared by bacteria other than E. coli.