Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters
Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters
复制标题
DOI：
10.1046/j.1365-2958.1998.00827.x
复制
发表时间：
1998-05-01
影响因子：
3.6
通讯作者：
Becker, JM
中科院分区：
文献类型：
--
作者：
Lubkowitz, MA;Barnes, D;Becker, JM
我们最近从白色念珠菌中克隆了一个寡肽转运基因，命名为 OPT1。该基因与之前没有确定功能的三个开放阅读框 (ORF) 显示出显着的序列相似性：在基因组计划中鉴定的来自粟酒裂殖酵母和酿酒酵母 YJL212C 和 YPR194C 的 isp4。 S. pombe 基因 isp4 最初由 Sate 等人鉴定。作为通过减数分裂的氮饥饿诱导上调的基因。然而，isp4 Delta 菌株在性分化方面表现出野生型表型。我们发现相同的 isp4 Delta 菌株缺乏四肽转运活性，这通过其对有毒四肽的抗性、无法积累放射性标记的四肽以及无法使用四肽作为氨基酸的唯一来源来测量。满足营养缺陷型的要求。同样，我们发现来自酿酒酵母的 ORF YPR194C 编码寡肽转运蛋白。序列分析和生理证据使我们提出，isp4 编码的蛋白质以及从酿酒酵母和白色念珠菌中鉴定的基因组成了一组新的小寡肽特异性转运蛋白，我们将其命名为 OPT 家族。
We have recently cloned an oligopeptide transport gene from Candida albicans denoted OPT1. This gene showed significant sequence similarity to three open reading frames (ORFs) with no previously established function: isp4 from Schizosaccharomyces pombe and Saccharomyces cerevisiae YJL212C and YPR194C, identified during the genome project. The S. pombe gene isp4 was originally identified by Sate ef al. as a gene that was upregulated through nitrogen starvation induction of meiosis. However, an isp4 Delta strain exhibited a wild-type phenotype with respect to sexual differentiation. We have found that the same isp4 Delta strain is deficient in tetrapeptide transport activity as measured by its resistance to toxic tetrapeptides, by its inability to accumulate a radiolabelled tetrapeptide and by the inability to use tetrapeptides as a sole source of an amino acid to. satisfy an auxotrophic requirement. Similarly, we found that the ORF YPR194C from S. cerevisiae encodes an oligopeptide transporter. Sequence analyses as well as physiological evidence has led us to propose that the proteins encoded by isp4 and the genes identified from S. cerevisiae and C. albicans comprise a new group of transporters specific for small oligopeptides, which we have named the OPT family.