MUTATION IN A STRUCTURAL GENE FOR A BETA-TUBULIN SPECIFIC TO TESTIS IN DROSOPHILA-MELANOGASTER
MUTATION IN A STRUCTURAL GENE FOR A BETA-TUBULIN SPECIFIC TO TESTIS IN DROSOPHILA-MELANOGASTER
复制标题
DOI：
10.1073/pnas.76.8.3991
复制
发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
RAFF, EC
中科院分区：
文献类型：
--
作者：
KEMPHUES, KJ;RAFF, RA;RAFF, EC
利用二维凝胶电泳技术对黑腹龙舌兰组织中制备的微管蛋白进行了分析。-微管蛋白亚基仅存在于睾丸中。在结构基因中的第3染色体显性突变[ms(3)KKD]。-微管蛋白被分离为雄性不育。这种突变的雄性杂合精子不能产生活动精子。从减数分裂开始，精子发生的所有过程都在一定程度上异常。许多微管（包括轴突的细胞质微管和双管）在横切面上显示出结构异常，发育中的精子的整体形态是紊乱的。通过二维凝胶电泳显示，这些男性的睾丸含有正常睾丸特异性的β。-微管蛋白和这种微管蛋白的电泳变体数量相等。野生型和突变型都是睾丸特异性的。-微管蛋白通过硫酸长春碱沉淀、与纯化果蝇胚胎微管蛋白共组装和肽定位鉴定。
By 2-dimensional gel electrophoresis of tubulins prepared from tissues of D. melanogaster a .beta.-tubulin subunit present only in the testis was identified. A 3rd chromosome dominant mutation [ms(3)KKD] in the structural gene for this .beta.-tubulin was isolated as a male sterile. Males heterozygous for this mutation produce no motile spermatozoa. Beginning with meiosis, all processes in spermiogenesis are abnormal to some extent. Many microtubules (including both cytoplasmic microtubules and doublet tubules of the axoneme) show aberrant structure in cross section, and the overall morphology of the developing spermatids is disorganized. Testes from these males were shown, by 2-dimensional gel electrophoresis, to contain both the normal testis-specific .beta.-tubulin and an electrophoretic variant of this tubulin in equal amounts. Both wild-type and mutant testis-specific .beta.-tubulins were characterized by vinblastine sulfate precipitation, coassembly with purified Drosophila embryo tubulin and peptide mapping.