Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.
Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.
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DOI：
10.1038/nbt.3594
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发表时间：
2016-08
影响因子：
46.9
通讯作者：
Smith SL
中科院分区：
文献类型：
--
作者：
Stirman JN;Smith IT;Kudenov MW;Smith SL
双光子钙成像提供了亚细胞分辨率的神经元群体中神经元活动的光学读数。然而，传统的双光子成像系统将视场限制在~1mm2，无法同时显示多个大脑皮层区域。在这里，我们展示了一个双光子显微镜与一个扩大的视野(>；9.5mm2)，以快速可重构的同时扫描广泛分离的神经元群体。我们定制设计并组装了一个优化的扫描引擎、OBJECT和两个可独立定位的、临时多路复用的激励路径。我们使用这种新的显微镜来测量小鼠在视觉处理过程中两个皮质视觉区域之间的活动相关性。
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ~1 mm2, precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm2) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.