Antifouling activities of hymenialdisine and debromohymenialdisine from the sponge Axinella sp.
Antifouling activities of hymenialdisine and debromohymenialdisine from the sponge Axinella sp.
复制标题
海绵 Axinella sp 中的 Hymenialdisine 和 debromohymenialdisine 的防污活性。
DOI：
10.1016/j.ibiod.2013.08.014
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发表时间：
2013-11-01
影响因子：
4.8
通讯作者：
Ke, Cai Huan
中科院分区：
文献类型：
--
作者：
Feng, Dan Qing;Qiu, Yan;Ke, Cai Huan
作为一种无物理保护的无底生物，海洋海绵被认为通过使用抗污次生代谢物来保护自己免受表面污染。本研究采用甲醇提取海绵Axinella sp.，然后在有机溶剂和水之间进行分离。采用Sephadex LH-20和C-18柱层析从正丁醇层中分离得到两个主要化合物：hymenialdisine （HD）和debromohymenialdisine （DBH）。通过绿贻贝产丝试验、苔藓虫幼虫和绿藻增生Ulva孢子的沉降试验，评价了HD和DBH的防污活性。HD和DBH对绿假单胞菌（P. viridis， EC50值分别为31.77和138.18 μ g ml(-1)）、黑螺旋藻（B. neritina， EC50值分别为3.43和8.17 μ g ml(-1)）和浒苔（U. prolifera， EC50值分别为8.31和0.67 μ g ml(-1)）具有显著的防污活性。我们的研究结果表明，HD和DBH可能在Axinella sp.对污染的化学防御中发挥作用。版权所有。
Being physically unprotected sessile organisms, marine sponges are thought to protect themselves from surface fouling through the use of antifouling secondary metabolites. In this study, the sponge Axinella sp. was extracted with methanol and then partitioned between organic solvents and water. Two main compounds, hymenialdisine (HD) and debromohymenialdisine (DBH), were isolated from the n-BuOH layer using Sephadex LH-20 and C-18 column chromatography. The antifouling activity of HD and DBH were evaluated using the test of byssus thread production with the green mussel Perna viridis, and the settlement assays with the bryozoan Bugula neritina larvae and the green alga Ulva prolifera spores. Both HD and DBH were found to exhibit significant antifouling activities against P. viridis (EC50 values of 31.77 and 138.18 mu g ml(-1), respectively), B. neritina (EC50 values of 3.43 and 8.17 mu g ml(-1), respectively) and U. prolifera (EC50 values of 8.31 and 0.67 mu g ml(-1), respectively). Our results suggested that HD and DBH may play a role in chemical defense against fouling in Axinella sp. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.