In vivo fluorescent imaging of the mouse retina using adaptive optics
In vivo fluorescent imaging of the mouse retina using adaptive optics
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DOI：
10.1364/ol.32.000659
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发表时间：
2007-03-15
期刊：
影响因子：
3.6
通讯作者：
Lin, Charles P.
中科院分区：
文献类型：
--
作者：
Biss, David P.;Sumorok, Daniel;Lin, Charles P.
由于眼睛引起的波前像差，使用可见光和近红外波长的小鼠视网膜的体内成像不能实现衍射极限分辨率。考虑到眼睛的瞳孔大小和轴向尺寸，预期视网膜的无像差成像将具有2 μ m的横向分辨率。自适应光学可以补偿人眼视网膜成像中的高阶像差。我们展示了一个自适应光学系统在体内成像的荧光结构在视网膜的小鼠，使用微机电系统膜镜和夏克哈特曼波前传感器，检测荧光波前。(c)2007年，美国光学学会。
In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of 2 mu m. Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack-Hartmann wavefront sensor that detects fluorescent wavefront. (c) 2007 Optical Society of America.