Closer look at the operating definition of protein recovery in CE.
Closer look at the operating definition of protein recovery in CE.
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仔细看看 CE 中蛋白质回收的操作定义。
DOI：
10.1002/elps.201200514
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发表时间：
2013
期刊：
影响因子：
2.9
通讯作者：
Sandoval,JuniorE
中科院分区：
文献类型：
--
作者：
Espinal,JoseH;Gomez,JorgeE;Sandoval,JuniorE
分析物回收率是评估熔融石英毛细管上蛋白质吸附的重要数据。 1991 年，Regnier 等人。通过假设分析物因吸附而损失以及因此由两个检测器测量的峰面积的减少与它们之间的毛细管部分的长度成正比来估计回收率。在本报告中，我们仔细研究了这一概念及其对商业 CE 仪器的适应，以确定蛋白质回收率。我们假设，一旦达到稳态迁移，蛋白质吸附就蛋白质浓度和吸附位点的表面密度而言是一级过程。该假设被证明在相当宽的毛细管有效长度范围内是有效的，因此，蛋白质回收率随着迁移距离呈指数下降。然而，与通过传统加标过程获得的传统回收率数据不同，通过这种方法测量的蛋白质回收率不具有相同的优点，因为它强烈依赖于毛细管尺寸和施加的电场。然而，蛋白质回收率和对数蛋白质峰面积与长度图的斜率是比较不同毛细管表面上蛋白质吸附的有用数据。讨论了一些涉及应用 Regnier 概念计算蛋白质回收率的文献报告。
Analyte recovery is an important figure to assess protein adsorption on fused‐silica capillaries. In 1991, Regnier et al. estimated recovery by assuming the loss of analyte from adsorption and thus the decrease in peak area measured by two detectors to be proportional to the length of the capillary section between them. In this report, we closely examine this concept and its adaptation to commercial CE instruments to determine protein recovery. We hypothesize that, once a steady‐state migration is reached, protein adsorption is a first‐order process with respect to protein concentration and surface density of adsorbing sites. This hypothesis is shown to be valid over a reasonably wide range of capillary effective length and, as a result, protein recovery decreases exponentially with the migrated distance. However, unlike the traditional recovery figure obtained through a conventional spike process, protein recovery measured by this approach does not have the same merit since it is strongly dependent from capillary dimensions and applied electric field. Nevertheless, protein recovery and the slope of the logarithmic protein peak area versus length plot are useful figures to compare protein adsorption on different capillary surfaces. Several literature reports dealing with the application of Regnier concept to calculate protein recovery are discussed.