The oral microbial composition and diversity affect the clinical course of palmoplantar pustulosis patients after dental focal infection treatment
The oral microbial composition and diversity affect the clinical course of palmoplantar pustulosis patients after dental focal infection treatment
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DOI：
10.1016/j.jdermsci.2021.11.001
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发表时间：
2021-12-13
影响因子：
4.6
通讯作者：
Kouno, Michiyoshi
中科院分区：
文献类型：
--
作者：
Akiyama, Yurie;Minabe, Masaki;Kouno, Michiyoshi
背景：掌跖脓疱病（PPP）是一种发生于手掌和脚掌的慢性脓疱性皮肤病。牙齿局灶性感染被认为是PPP的主要恶化因素。最近我们对口腔微生物组的研究表明PPP患者存在生态失调。虽然近一半的PPP患者在治疗后的牙齿局灶性感染改善，一定数量的患者并没有改善。目的：探讨口腔微生物因素影响PPP治疗后的牙齿局灶性感染的临床进程。方法：健康对照的口腔微生物群（n = 10），通过细菌16S核糖体RNA基因测序分析改善（n = 7）和未改善（n = 6）的患者。UniFrac分析表明改善和未改善患者之间的口腔微生物群差异。变形菌门的患病率在改善的患者中低于未改善的患者。当通过Shannon指数、Pielou指数和平均操作分类单位（OTU）评估α微生物多样性时，与改善的患者相比，未改善的患者具有较低的微生物群多样性。治疗后口腔菌群变化程度好转组高于未好转组。6个菌属与PPP患者的血液检测数据显示出显著相关性。结论：我们的研究结果表明，口腔微生物的组成和多样性可以解释PPP患者治疗后的牙局灶性感染的不同临床过程。PPP患者的口腔微生物组分析可能为牙科局灶性感染治疗的临床反应性提供预测因素。(C)2021日本皮肤病研究学会。Elsevier B.V.出版，保留所有权利。
Background: Palmoplantar pustulosis (PPP) is a chronic pustular dermatosis on the palms and soles. Dental focal infections are known as the major worsening factor for PPP. Recent our study of oral microbiome demonstrated dysbiosis in PPP patients. While almost half of the PPP patients improved after treatment of dental focal infections, a certain number of patients did not improve.Objective: To investigate the oral microbial factors affecting the clinical course of PPP after treatment of dental focal infection.Methods: The oral microbiota of healthy controls (n = 10), improved (n = 7) and not-improved (n = 6) patients were analyzed by sequencing of bacterial 16S ribosomal RNA gene.Results: The UniFrac analysis suggested the differences of oral microbiota between improved and not-improved patients. The prevalence of the phylum Proteobacteria was lower in improved patients than in notimproved patients. When the alpha microbial diversity was assessed by Shannon index, Pielou's index and the average operational taxonomic units (OTUs), not-improved patients had a lower-diversity microbiota compared to improved patients. The degree of changes of oral microbiota after dental focal infection treatment was higher in improved patients than in not-improved patients. Six genera showed significant correlation with blood test data of PPP patients.Conclusion: Our findings suggested that oral microbial compositions and diversity could account for the distinct clinical course of PPP patients after treatment of dental focal infection. Oral microbiome analysis of PPP patients may provide a predictive factor for clinical responsiveness to dental focal infection treatment. (C) 2021 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.