INFORMATION-CONTENT OF BINDING-SITES ON NUCLEOTIDE-SEQUENCES
INFORMATION-CONTENT OF BINDING-SITES ON NUCLEOTIDE-SEQUENCES
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DOI：
10.1016/0022-2836(86)90165-8
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发表时间：
1986-04-05
影响因子：
5.6
通讯作者：
EHRENFEUCHT, A
中科院分区：
文献类型：
--
作者：
SCHNEIDER, TD;STORMO, GD;EHRENFEUCHT, A
阻遏物、聚合酶、核糖体和其他大分子与特定的核酸序列结合。只有当序列具有可识别的模式时，它们才能找到结合位点。我们定义了一个衡量的信息（Rsequence）在结合位点的序列模式。它允许人们调查信息如何在站点之间分布，并将一个站点与另一个站点进行比较。我们还可以计算定位这些位点所需的信息量（R频率），因为它们在基因组中以一定的频率出现。几个大肠杆菌结合位点进行了分析，使用这两个独立的经验测量。对于我们分析的大多数网站，这两种信息量是相似的。相比之下，噬菌体T7 RNA聚合酶结合位点包含的信息大约是T7聚合酶识别所需信息的两倍，这表明第二种蛋白质可能与T7启动子结合。额外的信息可以解释为在T7启动子中发现的强对称元素。该元素可以是操作符。如果这个模型是正确的，那么这些发起人和运营商并没有分享太多信息。Rsequence和Rfrequency之间的比较表明，结合位点的信息足以将这些位点与基因组的其余部分区分开来。
Repressor, polymerases, ribosomes and other macromolecules bind to specific nucleic acid sequences. They can find a binding site only if the sequence has a recognizable pattern. We define a measure of the information (Rsequence) in the sequence patterns at binding sites. It allows one to investigate how information is distributed across the sites and to compare one site to another. One can also calculate the amount of information (Rfrequency) that would be required to locate the sites, given that they occur with some frequency in the genome. Several Escherichia coli binding sites were analyzed using these two independent empirical measurements. The two amounts of information are similar for most of the sites we analyzed. In contrast, bacteriophage T7 RNA polymerase bindings sites contain about twice as much information as is necessary for recognition by the T7 polymerase, suggesting that a second protein may bind at T7 promoters. The extra information can be accounted for by a strong symmetry element found at the T7 promoters. This element may be an operator. If this model is correct, these promoters and operators do not share much information. The comparisons between Rsequence and Rfrequency suggest that the information at binding sites is just sufficient for the sites to be distinguished from the rest of the genome.