Anti-obesity effect of Morus bombycis root extract: Anti-lipase activity and lipolytic effect
Anti-obesity effect of Morus bombycis root extract: Anti-lipase activity and lipolytic effect
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DOI：
10.1016/j.jep.2010.05.053
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发表时间：
2010-08-09
影响因子：
5.4
通讯作者：
Kim, Jin Sook
中科院分区：
文献类型：
--
作者：
Kim, Young Sook;Lee, Yun Mi;Kim, Jin Sook
研究目的：本研究评价了桑叶根乙醇提取物对脂肪细胞和脂肪组织中脂肪酶活性和脂解的抗肥胖作用。材料和方法：3.1.1.3通过在405 nm处测量对硝基苯基丁酸酯水解为对硝基苯酚来测定脂肪酶（三酰甘油酰基水解酶，EC www.example.com）活性。在完全分化的3 T3-L1脂肪细胞和脂肪组织中测定脂解作用。结果：家蚕根提取物具有较强的抗脂肪酶活性，IC_（50）值为2.07 μ g/mL。在分化的脂肪细胞和脂肪组织中，家蚕根提取物可增加脂肪分解作用，如降低细胞内甘油三酯和甘油的释放。此外，提取物抑制PDE活性的剂量依赖性marticle.Conclusion：本研究表明，Mows蚕蛾根提取物可能是治疗肥胖症的治疗方面的利益。皇冠版权所有（C）2010由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Aim of the study: This study evaluated anti-obesity effect of the ethanolic extract of Morus bombycis root on lipase activity and lipolysis in adipocytes and adipose tissues.Materials and methods: Lipase (triacylgycerol acylhydrolase, EC 3.1.1.3) activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Lipolytic effects were assayed in fully differentiated 3T3-L1 adipocytes and adipose tissues. In vitro, phosphodiesterase (PDE) activity was also measured.Results: Mows bombycis root extract exhibited strong anti-lipase activity, with an IC50 value of 2.07 mu g/mL In differentiated adipocytes and adipose tissues, the extract increased lipolytic effects such as decreased intracellular triglyceride and the release of glycerol. Further, the extract inhibited PDE activity in a dose-dependent manner.Conclusion: The present study suggests that Mows bombycis root extract might be of therapeutic interest with respect to the treatment of obesity. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved.