Hepatitis B viral antigenic structure: signature analysis by monoclonal radioimmunoassays.
Hepatitis B viral antigenic structure: signature analysis by monoclonal radioimmunoassays.
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乙型肝炎病毒抗原结构：单克隆放射免疫分析的特征分析。
DOI：
10.1073/pnas.81.7.2237
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发表时间：
1984
影响因子：
11.1
通讯作者：
Isselbacher,KJ
中科院分区：
文献类型：
--
作者：
Wands,JR;Wong,MA;Shorey,J;Brown,RD;Marciniak,RA;Isselbacher,KJ
已经开发了一种方法来分析乙型肝炎病毒（HBV）抗原结构，该结构可以创建HBV毒株的数字“签名”。该技术采用高亲和力的IgM和IgG单克隆抗体（抗HBsAg），针对乙型肝炎表面抗原（HBsAg）的不同决定因素。这些抗体已被用于开发敏感和特异性的放射免疫测定血清中hbsag相关决定因素的测定。在进行“签名”分析时，通过测量连续稀释的hbsag阳性血清的免疫反应性，生成每个单克隆抗hbsag的单独结合曲线。由于血清中HBsAg浓度未知，因此通过迭代最大似然程序对来自同一“经典”HBV亚型的样品组的结合谱进行对齐，以给出该HBV亚型的数值特征。通过使用这种方法，HBsAg显示出比先前多价抗HBsAg抗体更多的抗原异质性。事实上，在经典的HBsAg亚型中还有亚群。此外，a结构域（所有已知HBV亚型或毒株共有）已被证明是多决定因素。因此，这些研究证明了迄今为止未被认识到的HBV亚型差异。由于不需要知道复杂蛋白混合物中病毒或病毒蛋白的浓度，该方法对其他病毒制剂之间细微或主要抗原变化的研究也具有更广泛的意义。
An approach has been developed for the analysis of hepatitis B viral (HBV) antigenic structure that creates numerical "signatures" of HBV strains. This technique employs high-affinity IgM and IgG monoclonal antibodies (anti-HBsAg) directed toward distinct and separate determinants on hepatitis B surface antigen (HBsAg). Such antibodies have been used to develop sensitive and specific radioimmuno-assays for measurement of HBsAg-associated determinants in serum. In performing "signature" analysis separate binding curves for each monoclonal anti-HBsAg are generated by measuring immunoreactivity in serial dilutions of HBsAg-positive serum. Since the HBsAg concentration in serum is unknown, the binding profiles of groups of samples from the same "classic" HBV subtype are aligned by an iterative maximum likelihood procedure to give the numerical signature of that HBV subtype. By using this approach, HBsAg shows far more antigenic heterogeneity than previously recognized by polyvalent anti-HBsAg antibodies. Indeed, there are subgroups within the classic HBsAg subtypes. In addition, the a domain (common to all known subtypes or strains of HBV) has been shown to be multideterminant. Thus, these studies have demonstrated heretofore unrecognized differences in HBV subtypes. This approach also has broader significance for the study of subtle or major antigenic changes among other viral agents since it is not necessary to know the concentration of virus or viral protein in complex protein mixtures.