PEX26 Functions as a Metastasis Suppressor in Colorectal Cancer
PEX26 Functions as a Metastasis Suppressor in Colorectal Cancer
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DOI：
10.1007/s10620-023-08168-w
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发表时间：
2023-11
影响因子：
3.1
通讯作者：
Bianbian Yan;Lichao Cao;Liyang Gao;Shangqing Wei;Mengwei Wang;Ye Tian;Jin Yang;E. Chen
中科院分区：
文献类型：
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作者：
Bianbian Yan;Lichao Cao;Liyang Gao;Shangqing Wei;Mengwei Wang;Ye Tian;Jin Yang;E. Chen
背景/目的异常过氧化物酶体生物发生因子26（PEX 26）存在于多细胞过程中。然而，PEX 26在结直肠癌（CRC）发展中的作用仍然未知。本研究旨在探讨PEX 26在结直肠癌细胞中的表达、调控及功能。方法采用生物信息学分析、实时荧光定量PCR及免疫组化方法检测PEX 26在结直肠癌及正常组织中的表达。我们进行了体外功能实验以阐明PEX 26对CRC细胞的作用。结果PEX 26在结直肠癌中表达下调，其低表达与结直肠癌患者的生存率相关。我们进一步证明了PEX 26过表达抑制CRC细胞迁移、侵袭和上皮-间质转化（EMT）的能力，而PEX 26敲低通过激活Wnt通路促进CRC细胞迁移、侵袭和EMT的恶性表型。PEX 26可能是一种新的结直肠癌转移抑制因子。
Background/AimsAberrant Peroxisomal Biogenesis Factor 26 (PEX26) occurs in multiple cell process. However, the role of PEX26 in colorectal cancer (CRC) development remains unknown. We aimed to study PEX26 expression, regulation, and function in CRC cells.MethodsUsing the bioinformatic analysis, real-time quantitative PCR, and immunohistochemistry staining, we detected the expression of PEX26 in CRC and normal tissues. We performed functional experiments in vitro to elucidate the effect of PEX26 on CRC cells. We analyzed the RNA-seq data to reveal the downstream regulating network of PEX26.ResultsPEX26 is significantly down-regulated in CRC and its low expression correlates with the poor overall survival of CRC patients. We further demonstrated that PEX26 over-expression inhibits the ability of CRC cell migration, invasion, and epithelial–mesenchymal transition (EMT), while PEX26 knockdown promotes the malignant phenotypes of migration, invasion, and EMT via activating the Wnt pathway.ConclusionOverall, our results showed that the loss of PEX26 contributes to the malignant phenotype of CRC. PEX26 may serve as a novel metastasis repressor for CRC.