Divergence in plasmatic and urinary isoprostane levels in type 2 diabetes
Divergence in plasmatic and urinary isoprostane levels in type 2 diabetes
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DOI：
10.1016/s0009-8981(02)00213-9
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发表时间：
2002-10-01
影响因子：
5
通讯作者：
Mazur, A
中科院分区：
文献类型：
--
作者：
Feillet-Coudray, C;Choné, F;Mazur, A
背景：氧化应激目前被认为是糖尿病的一种机制。本研究旨在评估2型糖尿病患者血浆和尿液中的异前列腺素水平，并将其与目前使用的其他氧化应激生物标志物进行比较。方法：选取对照组（n = 10）和2型糖尿病组（n = 10）。除了传统的生化参数外，我们还评估了血浆和尿液中异前列腺素和丙二醛（MDA）水平作为氧化应激的标志物。结果：糖尿病患者血浆和尿丙二醛升高，血浆维生素e几乎显著降低。糖尿病患者尿异前列腺素水平升高，但血浆异前列腺素有较强的下降趋势。因此，在研究的糖尿病患者中，尽管体内异前列腺素的产生增加（因为其他血浆氧化应激生物标志物被改变），但它并未导致血浆异前列腺素水平的增加。可以假设，这是由于这种代谢物的消除增加，因此尿液中的排泄增加。结论：我们的研究结果表明，在血浆和尿液这两种不同的生物体液中测量相同的氧化应激生物标志物异前列腺素，会导致不同的结果，并强调了在循环液（血浆）和消除液（尿液）中测量生物标志物的重要性，以便对生物体中发生的事情有一个大致的了解。(C) 2002 Elsevier Science B.V.版权所有
Background: Oxidative stress is currently suggested as a mechanism underlying diabetes. The present study was designed to evaluate isoprostane levels in plasma and in urine in type 2 diabetic patients, and to compare them to other currently used biomarkers of oxidative stress. Methods: The work was performed in a control group (n = 10) and in a type 2 diabetic group (n = 10). Besides the traditional biochemical parameters, we evaluated the plasma and urine levels of isoprostanes and malondialdehyde (MDA) as markers of oxidative stress. Results: We found increased plasma and urine MDA in the diabetic patients and almost significantly decreased plasma vitamin E. Urinary isoprostane levels in diabetic patients were increased but they presented a strong tendency to a decrease in plasma isoprostanes. It is therefore suggested that, in the studied diabetic patients, although the production of isoprostanes in the body was increased (as other plasma oxidative stress biomarkers were altered) it did not lead to an increase in plasma isoprostane levels. It could be hypothesised that this results from an increased elimination of this metabolite and therefore an increased excretion in urine. Conclusion: Our results showed that the measurement of same oxidative stress biomarker, isoprostane, in two different biologic fluids, plasma and urine, led to divergent results and emphasised the importance to measure a biomarker both in the circulation fluid (plasma) and in the elimination fluid (urine), to have a general idea of what is occurring in the organism. (C) 2002 Elsevier Science B.V. All rights reserved.