The epsilon subunit of DNA polymerase III Is involved in the nalidixic acid-induced SOS response in Escherichia coli.
The epsilon subunit of DNA polymerase III Is involved in the nalidixic acid-induced SOS response in Escherichia coli.
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DNA 聚合酶 III 的 ε 亚基参与大肠杆菌中萘啶酸诱导的 SOS 反应。
DOI：
10.1128/jb.00173-08
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发表时间：
2008
影响因子：
3.2
通讯作者：
Kreuzer,KennethN
中科院分区：
文献类型：
--
作者：
Pohlhaus,JenniferReineke;Long,DavidT;O'Reilly,Erin;Kreuzer,KennethN
Quinolone antibacterial drugs such as nalidixic acid target DNA gyrase inEscherichia coli. These inhibitors bind to and stabilize a normally transient covalent protein-DNA intermediate in the gyrase reaction cycle, referred to as the cleavage complex. Stabilization of the cleavage complex is necessary but not sufficient for cell killing—cytotoxicity apparently results from the conversion of cleavage complexes into overt DNA breaks by an as-yet-unknown mechanism(s). Quinolone treatment induces the bacterial SOS response in a RecBC-dependent manner, arguing that cleavage complexes are somehow converted into double-stranded breaks. However, the only proteins known to be required for SOS induction by nalidixic acid are RecA and RecBC. In hopes of identifying additional proteins involved in the cytotoxic response to nalidixic acid, we screened forE. colimutants specifically deficient in SOS induction upon nalidixic acid treatment by using adinD::lacZreporter construct. From a collection of SOS partially constitutive mutants with disruptions of 47 different genes, we found thatdnaQinsertion mutants are specifically deficient in the SOS response to nalidixic acid.dnaQencodes DNA polymerase III ε subunit, the proofreading subunit of the replicative polymerase. The deficient response to nalidixic acid was rescued by the presence of the wild-typednaQgene, confirming involvement of the ε subunit. To further characterize the SOS deficiency ofdnaQmutants, we analyzed the expression of several additional SOS genes in response to nalidixic acid using real-time PCR. A subset of SOS genes lost their response to nalidixic acid in thednaQmutant strain, while two tested SOS genes (recAandrecN) continued to exhibit induction. These results argue that the replication complex plays a role in modulating the SOS response to nalidixic acid and that the response is more complex than a simple on/off switch.
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静脉注射钆-DTPA 增强颈椎管内肿瘤的 MR 成像
影响因子：
1.3
作者：
G. M. Bydder;J. Brown;Niendorf Hp;Young Ir
通讯作者：
Young Ir
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影响因子：
4.1
作者：
G. Schroth;A. Thron;L. Guhl;K. Voigt;H. Niendorf;L. R. Garces
通讯作者：
L. R. Garces
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影响因子：
19.7
作者：
E. Russell;C. M. D'Angelo;R. Zimmerman;L. Czervionke;M. Huckman
通讯作者：
M. Huckman
脊柱髓内疾病：使用钆-DTPA 增强 MR 成像进行诊断。
DOI：
--
发表时间：
1988
期刊：
AJR. American journal of roentgenology
影响因子：
--
作者：
G. Sze;G. Krol;Robert D. Zimmerman;M. F. Deck
通讯作者：
M. F. Deck
脊柱肿瘤的钆-DTPA 增强 MR 成像：初步研究以及与未增强自旋回波和 STIR 序列的比较。
DOI：
10.2214/ajr.151.6.1185
发表时间：
1988
期刊：
AJR. American journal of roentgenology
影响因子：
--
作者：
G. Stimac;BRUCE A. Porter;Dana O. Olson;Rebecca Gerlach;Rebecca Gerlach2;Monique Genton
通讯作者：
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