Quantitative profiling of peptides from RNAs classified as noncoding.
Quantitative profiling of peptides from RNAs classified as noncoding.
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DOI：
10.1038/ncomms6429
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发表时间：
2014-11-18
影响因子：
16.6
通讯作者：
Steen JA
中科院分区：
文献类型：
--
作者：
Prabakaran S;Hemberg M;Chauhan R;Winter D;Tweedie-Cullen RY;Dittrich C;Hong E;Gunawardena J;Steen H;Kreiman G;Steen JA
Only a small fraction of the mammalian genome codes for messenger RNAs destined to be translated into proteins, and it is generally assumed that a large portion of transcribed sequences - including introns and several classes of non-coding RNAs (ncRNAs) do not give rise to peptide products. A systematic examination of translation and physiological regulation of ncRNAs has not been conducted. Here, we use computational methods to identify the products of non-canonical translation in mouse neurons by analyzing unannotated transcripts in combination with proteomic data. This study supports the existence of non-canonical translation products from both intragenic and extragenic genomic regions, including peptides derived from anti-sense transcripts and introns. Moreover, the studied novel translation products exhibit temporal regulation similar to that of proteins known to be involved in neuronal activity processes. These observations highlight a potentially large and complex set of biologically regulated translational events from transcripts formerly thought to lack coding potential.
