Improved LC/MS Methods for the Analysis of Metal-Sensitive Analytes Using Medronic Acid as a Mobile Phase Additive
Improved LC/MS Methods for the Analysis of Metal-Sensitive Analytes Using Medronic Acid as a Mobile Phase Additive
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DOI：
10.1021/acs.analchem.8b02100
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发表时间：
2018-08-07
影响因子：
7.4
通讯作者：
Yin, Hongfeng
中科院分区：
文献类型：
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作者：
Hsiao, Jordy J.;Potter, Oscar G.;Yin, Hongfeng
在LC/MS实验中，通常观察到磷酸化化合物和具有多个羧酸酯基团的有机酸具有差的峰形和信号。不良峰形是由于色谱系统内存在多种来源的痕量金属（特别是铁）所致。为了改善这个问题，研究了不同的溶剂添加剂以减少流动路径中的金属量，从而对这些金属敏感化合物实现更好的分析性能。在这里，我们介绍了溶剂添加剂的使用，可以显着改善LC/MS分析的金属敏感代谢物的峰形和信号。此外，添加剂被证明是适合于其他金属敏感的应用，如磷酸肽和极性磷酸化农药的分析，其中的仪器可以用于阳性或阴性分析模式。
Phosphorylated compounds and organic acids with multiple carboxylate groups are commonly observed to have poor peak shapes and signal in LC/MS experiments. The poor peak shape is caused by the presence of trace metals, particularly iron, contributed from a variety of sources within the chromatographic system. To ameliorate this problem, different solvent additives were investigated to reduce the amount of metal in the flow path to achieve better analytical performance for these metal-sensitive compounds. Here, we introduce the use of a solvent additive that can significantly improve the peak shapes and signal of metal sensitive metabolites for LC/MS analysis. Moreover, the additive is shown to be amenable for other metal-sensitive applications, such as the analysis of phosphopeptides and polar phosphorylated pesticides, where the instruments could be used in either positive or negative analysis mode.