Microwave irradiated collagen tubes as a better matrix for peripheral nerve regeneration
Microwave irradiated collagen tubes as a better matrix for peripheral nerve regeneration
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DOI：
10.1016/j.brainres.2005.03.022
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发表时间：
2005-06-07
期刊：
影响因子：
2.9
通讯作者：
Jayakumar, R
中科院分区：
文献类型：
--
作者：
Ahmed, MR;Vairamuthu, S;Jayakumar, R
胶原蛋白具有良好的生物相容性和拉伸强度，是制备神经导引管的最佳材料之一。在这项工作中，我们比较了再生和功能性神经再支配后，坐骨神经切除与生物可吸收交联胶原蛋白指南在10毫米的差距。用戊二醛（GTA）或微波辐射（MWI）进行交联。采用薄层蒸发法制备了用于神经导引的多层胶原膜。通过测量坐骨神经功能指数（SFI）、神经传导速度（NCV）和肌电图（EMG）对再生神经进行功能评价。透射电子显微镜研究显示，植入1个月后，未交联胶原管治疗组的轴突电缆生长，有髓轴突、雪旺细胞较少，无髓轴突较多。然而，在相同的植入时间段后，我们观察到在自体移植物、GTA和MWI交联胶原蛋白管植入物的情况下，在1 cm的差距中有更多的有髓鞘轴突。与自体移植物和MWI胶原管组相比，GTA交联胶原管组的有髓纤维直径较小。光镜观察显示，术后3个月和6个月，再生神经中有更多的有髓轴突。与GTA和未交联胶原管相比，自体移植物和MWI交联胶原管组的传导速度和恢复指数在5个月后显著改善，达到正常值。(c)2005 Elsevier B.V.保留所有权利。
Collagen is one of the best materials used for nerve guide preparation due to its biocompatibility and desirable tensile strength. In this work, we have compared regeneration and functional reinnervation after sciatic nerve resection with bioresorbable crosslinked collagen guides in 10 mm gap. The crosslinking was carried out either with glutaraldehyde (GTA) or microwave irradiation (MWI). The multilayered collagen membrane used for nerve guides are prepared by lamellar evaporation technique. Functional evaluations of the regenerated nerves were performed by measuring the sciatic functional index (SFI), nerve conduction velocity (NCV), and electromyography (EMG). Transmission electron microscopic studies showed growth of axonal cable with fewer myelinated axons, Schwann cells and more unmyelinated axons present in the case of group treated with uncrosslinked collagen tubes after 1 month of implantation. However, we have observed more myelinated axons in the case of autograft, GTA, and MWI crosslinked collagen tube implants across the gap of 1 cm after the same period of implantation. Smaller myelinated fiber diameter was observed in the case of GTA crosslinked collagen tube group when compared with the autograft and MWI collagen tube groups. There were more myelinated axons during the 3rd and 6th months postoperatively using these conduits as substantiated by light microscopic studies of the regenerated nerve. The conduction velocity and recovery index improved significantly after 5 months reaching the normal values in the autograft and MWI crosslinked collagen groups compared to GTA and uncrosslinked collagen tubes. (c) 2005 Elsevier B.V. All rights reserved.